Modulation of adenovirus transformation by thyroid hormone.
Modulation of adenovirus transformation by thyroid hormone.
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甲状腺激素对腺病毒转化的调节。
DOI：
10.1073/pnas.80.1.196
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发表时间：
1983
影响因子：
11.1
通讯作者：
Edelman,IS
中科院分区：
文献类型：
--
作者：
Fisher,PB;Guernsey,DL;Weinstein,IB;Edelman,IS
我们已经研究了三碘甲状腺原氨酸（T3）的影响从头转化的克隆人口的Fischer大鼠胚胎成纤维细胞（CREF）的温度敏感的突变体（H5 ts 125）的5型腺病毒和在这些细胞中的转化表型的表达。当CREF细胞在感染H5 ts 125之前、期间和之后在缺乏T3的培养基中生长时，转化灶的产量是补充有1 nM T3的培养物中的一半。在转化过程的各个阶段中选择性地添加或去除T3表明，该激素在病毒感染后72小时内发挥其最大作用。T3也是先前由5型腺病毒wt-3A和ts-7 E转化的CREF细胞的两个克隆在琼脂中最佳生长所需的。肿瘤促进剂12-O-tetradecanoylphorbol 13-acetate可以取代T3在增强wt-3A但不是ts-7 E在琼脂中的生长，这表明启动子和T3通过不同的机制改变锚定非依赖性生长。正常的CREF细胞和两个转化的CREF克隆在含有或缺乏T3的培养基中的单层培养中同样生长良好。这两个转化的CREF克隆含有较低数量的核T3受体比CREF细胞，他们绑定的佛波醇二丁酸酯的水平略低。这些结果表明，甲状腺激素调节参与腺病毒转化的早期阶段，它也增强了先前转化细胞中转化状态的表达。
We have examined the effect of triiodothyronine (T3) on de novo transformation of a cloned population of Fischer rat embryo fibroblasts (CREF) by a temperature-sensitive mutant (H5ts125) of type 5 adenovirus and on the expression of the transformed phenotype in these cells. When CREF cells were grown in medium lacking T3 before, during, and after infection with H5ts125, the yield of transformed foci was half that in the cultures supplemented with 1 nM T3. Selective addition or removal of T3 during various phases of the transformation process indicated that the hormone exerted its maximal effect within 72 hr after viral infection. T3 was also required for optimal growth in agar of two clones of CREF cells previously transformed by type 5 adenovirus, wt-3A and ts-7E. The tumor promoter 12-O-tetradecanoylphorbol 13-acetate could substitute for T3 in enhancing growth in agar of wt-3A but not of ts-7E, suggesting that the promoter and T3 modify anchorage-independent growth by different mechanisms. Normal CREF cells and both of the transformed CREF clones grew equally well in monolayer culture in medium containing or lacking T3. Both of the transformed CREF clones contained a lower number of nuclear T3 receptors than did CREF cells and they bound somewhat lower levels of phorbol dibutyrate. These results indicate that thyroid hormone modulates an early stage involved in adenovirus transformation and that it also enhances the expression of the transformed state in previously transformed cells.