Antitumor effects of vaccine consisting of dendritic cells pulsed with tumor RNA from gastric cancer
Antitumor effects of vaccine consisting of dendritic cells pulsed with tumor RNA from gastric cancer
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DOI：
10.3748/wjg.v10.i5.630
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发表时间：
2004-03-01
影响因子：
4.3
通讯作者：
Lin, Yan-Zhen
中科院分区：
文献类型：
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作者：
Liu, Bing-Ya;Chen, Xue-Hua;Lin, Yan-Zhen
目的：探讨MFC胃癌细胞总RNA冲击的树突状细胞(DC)疫苗的免疫治疗作用。方法：从615系小鼠的脾组织中，用磁性细胞分选法(MACS)制备DC。培养24 h后，用MFC胃癌细胞总RNA冲击DC。其中一组小鼠于接种肿瘤前的第14天和第7天以1×10(6)的剂量接种肿瘤RNA脉冲DC(RNADC)。另一组小鼠以与RNA/DC组相同剂量的非冲击DC(UDC)免疫小鼠。第三组对照小鼠不接受治疗。在d0，所有小鼠均以S c注射5x10(5)MFC胃癌细胞进行攻击。接种后，对小鼠的肿瘤生长进行了密切监测。结果：接种肿瘤细胞后第21天，对照组小鼠肿瘤体积最大，平均体积为2.6323+/-1.1435 cm(3)，其次是UDC组和RNA/DC组，平均体积分别为0.7536+/-0.3659 cm(3)和0.3688+/-0.6571 cm(3)。RNA/DC组小鼠外周血和脾细胞中NK细胞活性分别比对照组高66.2%和65.4%。RNA/DC组的肿瘤特异性CTL活性为49.5%，高于对照组。结论：经胃癌细胞总RNA冲击的DC瘤苗体内接种具有刺激肿瘤特异性CTL活性和建立抗肿瘤免疫的能力。
AIM: To investigate the immunotherapeutic potential of vaccine consisting of dendritic cells (DCs) pulsed with total RNA from MFC gastric cancer cells.METHODS: DCs were prepared from the spleens of strain 615 mice by magnetic cell sorting (MACS). After culture for 24 h, DCs were pulsed with total RNA from MFC gastric cancer cells. Mice of one group were immunized with tumor RNA pulsed DC (RNA/DC) at the dosage of 1x10(6) on d 14 and 7 by s c inoculation before tumor implantation. Mice of another group were immunized with unpulsed DC (UDC) at the same dosage on days as the RNA/DC group. The third group of control mice was untreated. On d 0, all the mice were challenged with s c injections of 5x10(5) MFC gastric cancer cells. After inoculation, the mice were monitored closely with respect to tumor growth. Activities of NK cells in PBL and splenocytes and CTL were tested.RESULTS: On d 21 after tumor cell inoculation, the mice of control group manifested the largest tumors with volume at a mean of 2.6323 +/- 1.1435 cm(3), followed by the UDC and RNA/DC groups with mean volumes at 0.7536 +/- 0.3659 cm(3) and 0.3688 +/- 0.6571 cm(3), respectively. The activities of NK cells in PBL and splenocytes in RNA/DC group were 66.2% and 65.4%, respectively, higher than that in the control group. The tumor specific CTL activity in RNA/DC group was 49.5%, higher than that in the control group.CONCLUSION: The tumor vaccine with DCs pulsed with total RNA from gastric cancer cells possesses the ability to stimulate tumor specific CTL activity and to establish antitumor immunity when administered in vivo.