Role of the Intravitreal Growth Factors in the Pathogenesis of Idiopathic Epiretinal Membrane
Role of the Intravitreal Growth Factors in the Pathogenesis of Idiopathic Epiretinal Membrane
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DOI：
10.1167/iovs.10-7116
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发表时间：
2011-07-01
影响因子：
4.4
通讯作者：
Pivetti-Pezzi, Paola
中科院分区：
文献类型：
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作者：
Iannetti, Ludovico;Accorinti, Massimo;Pivetti-Pezzi, Paola
目的.本研究旨在探讨TGF β 1和TGF β 2、胶质细胞源性神经营养因子（GDNF）和神经生长因子（NGF）在特发性视网膜前膜（ERM）发病机制中的作用。本研究入组了8例受特发性ERM影响的患者，6例男性和2例女性，平均年龄为60.25 ± 17.16岁（范围：33-75岁）。所有患者均接受了标准的睫状体平坦部玻璃体切除术，并进行了膜去除和特异性ELISA，以评估玻璃体样本中的TGF β 1，TGF β 2，GDNF和NGF。在用盐酸酸化样品后重复该过程。在酸化前，ELISA分析显示特发性ERM样本中TGF β 2显著增加（327.98 +/- 99.58 pg/mL;范围：206.864-466.235 pg/mL）与对照组相比（187.17 +/- 58.20 pg/mL;范围，132.758-271.707 pg/mL; t = 3.4; P < 0.05）。酸化样品后也获得了统计学显著差异（618.15 +/- 201.43 pg/mL;范围409.795-866.215 pg/mL与265.04 +/- 98.15 pg/mL;范围152.478-352.101 pg/mL; t = 4.5; P < 0.05）。值得注意的是，酸化前两组之间的NGF差异无统计学意义（t = 0.79; P = 0.46），而酸化后，与对照组相比，发现ERM样品中的NGF水平显著增加（723.41 ± 235.4 vs. 242.84 ± 104.61; t = 3; P < 0.05）。目前的研究表明，TGF β 2和NGF与特发性ERMs相关，提示了一种迄今为止从未提出的新的代偿机制。（Invest Ophthalmol维斯科学。2011;52：5786-5789）DOI：10.1167/iovs.10-7116
PURPOSE. The aim of the present study is to evaluate the roles of TGFs beta 1 and beta 2, glial cell line-derived neurotrophic factor (GDNF), and nerve growth factor (NGF) in the pathogenesis of idiopathic epiretinal membrane (ERM).METHODS. Eight patients, six males and two females, with an average age of 60.25 +/- 17.16 years (range, 33-75 years) who were affected by idiopathic ERM were enrolled in the study. All patients underwent standard pars plana vitrectomy surgery with membrane removal and specific ELISA was performed to evaluate TGF beta 1, TGF beta 2, GDNF, and NGF in the vitreous samples. This was repeated after acidification of the samples with hydrochloric acid.RESULTS. Before acidification, ELISA analysis revealed a significant increase of TGF beta 2 in the samples with idiopathic ERM (327.98 +/- 99.58 pg/mL; range, 206.864-466.235 pg/mL) compared to the control group (187.17 +/- 58.20 pg/mL; range, 132.758-271.707 pg/mL; t = 3.4; P < 0.05). A statistically significant difference was also obtained after acidification of the samples (618.15 +/- 201.43 pg/mL; range, 409.795-866.215 pg/mL compared to 265.04 +/- 98.15 pg/mL; range, 152.478-352.101 pg/mL; t = 4.5; P < 0.05). Notably, before acidification the differences in NGF between the two groups were not statistically significant (t = 0.79; P = 0.46), while after acidification a significant increase of the NGF levels in ERM samples was found in comparison with the control group (723.41 +/- 235.4 vs. 242.84 +/- 104.61; t = 3; P < 0.05).CONCLUSIONS. The present study reveals that TGF beta 2 and NGF are associated with idiopathic ERMs, suggesting a novel compensatory mechanism so far never proposed. (Invest Ophthalmol Vis Sci. 2011;52:5786-5789) DOI:10.1167/iovs.10-7116