PLANAR INDUCTION OF ANTEROPOSTERIOR PATTERN IN THE DEVELOPING CENTRAL-NERVOUS-SYSTEM OF XENOPUS-LAEVIS
PLANAR INDUCTION OF ANTEROPOSTERIOR PATTERN IN THE DEVELOPING CENTRAL-NERVOUS-SYSTEM OF XENOPUS-LAEVIS
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DOI：
10.1126/science.1636091
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发表时间：
1992-07-24
期刊：
影响因子：
56.9
通讯作者：
GERHART, JC
中科院分区：
文献类型：
--
作者：
DONIACH, T;PHILLIPS, CR;GERHART, JC
长期以来人们认为，脊椎动物中枢神经系统的前后（A - P）模式是在胚胎的背外胚层中完全由从下方有模式的背中胚层垂直传递的信号所诱导的。制备了非洲爪蟾早期原肠胚的外植体，其中背外胚层和中胚层之间的垂直接触被阻止，但平面接触得以维持。在这些外植体中，四种位置特异性神经标记物（ engrailed - 2、Krox - 20、XIHbox 1和XIHbox 6）在外胚层中按照与胚胎中相同的A - P顺序表达。因此，仅平面信号沿着正常胚胎中可用的路径就能够诱导A - P神经模式。
It has long been thought that anteroposterior (A-P) pattern in the vertebrate central nervous system is induced in the embryo's dorsal ectoderm exclusively by signals passing vertically from underlying, patterned dorsal mesoderm. Explants from early gastrulae of the frog Xenopus laevis were prepared in which vertical contact between dorsal ectoderm and mesoderm was prevented but planar contact was maintained. In these, four position-specific neural markers (engrailed-2, Krox-20, XIHbox 1, and XIHbox 6) were expressed in the ectoderm in the same A-P order as in the embryo. Thus, planar signals alone, following a path available in the normal embryo, can induce A-P neural pattern.