Pericyte coverage of differentiated vessels inside tumor vasculature is an independent unfavorable prognostic factor for patients with clear cell renal cell carcinoma
Pericyte coverage of differentiated vessels inside tumor vasculature is an independent unfavorable prognostic factor for patients with clear cell renal cell carcinoma
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肿瘤脉管系统内分化血管的周细胞覆盖是透明细胞肾细胞癌患者的独立不利预后因素
DOI：
10.1002/cncr.27746
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发表时间：
2013-01-15
期刊：
影响因子：
6.2
通讯作者：
Qian, Chao-Nan
中科院分区：
文献类型：
--
作者：
Cao, Yun;Zhang, Zhi-Ling;Qian, Chao-Nan
背景。本研究的目的是评估分化肿瘤微血管周细胞覆盖率（PC）对透明细胞肾细胞癌（CCRCC）患者预后的影响。方法。来自两个CCRCC患者队列（101名亚洲患者和524名美国患者）的样本采用两种不同的组织学方法制备：常规切片和组织微阵列。然后，对样本进行免疫组织化学双染，检测周细胞标记物（α -平滑肌肌动蛋白[a- sma]）和分化血管标记物（分化集群34 [CD34]），然后进行多光谱图像捕获和计算机图像分析，量化微血管密度（MVD）和分化血管的PC。分析PC、MVD:PC比值与临床病理特征的相关性。结果。分化MVD与PC呈负相关。在两个队列中，较高的PC与CCRCC更具侵袭性的临床病理特征相关，包括更高级的t分类、更高的病理分级和肿瘤坏死的发生。MVD:PC比值是亚洲队列中总生存率和无复发生存率以及美国队列中无复发生存率的独立有利预后因素。PC也是一个独立的预后因素，较高的PC预示着较差的结果。结合PC和MVD能更好地区分CCRCC患者的预后。PC联合分化MVD或MVD:PC比值为Leibovich风险模型提供了额外的、独立的预后信息，这些信息用于生成改进的风险模型。结论。作者一致观察到，较高的PC与更具侵袭性的临床病理特征相关。PC是一个独立的不利预后因素。作者得出结论，周细胞应该作为治疗的靶点。2013年癌症。(c) 2012年美国癌症协会。
BACKGROUND. The objective of this study was to evaluate the effect of pericyte coverage (PC) of differentiated tumor microvessels on the prognosis of patients with clear cell renal cell carcinoma (CCRCC). METHODS. Samples from 2 cohorts of patients with CCRCC (101 Asian patients and 524 US patients) were prepared using 2 different histologic approaches: routine sectioning versus tissue microarray. Then, the samples were immunohistochemically doubled-stained for a pericyte marker (alpha smooth muscle actin [a-SMA]) and a differentiated vessel marker (cluster of differentiation 34 [CD34]), followed by multispectral image capturing and computerized image analyses to quantify the microvessel density (MVD) and the PC of differentiated vessels. The correlations of PC and the MVD:PC ratio with clinicopathologic characteristics were analyzed. RESULTS. There was an inverse correlation between differentiated MVD and PC. Higher PC correlated with more aggressive clinicopathologic characteristics of CCRCC in both cohorts, including more advanced T-classification, higher pathologic grades, and the occurrence of tumor necrosis. The MVD:PC ratio was an independent favorable prognostic factor for overall and recurrence-free survival in the Asian cohort and for recurrence-free survival in the US cohort. PC also was an independent prognostic factor, with higher PC predicting a poorer outcome. The combination of PC and MVD was better at distinguishing the outcome of patients with CCRCC. PC combined with differentiated MVD or with the MVD:PC ratio provided additional, independent prognostic information to the Leibovich risk model, and that information was used to generate improved risk models. CONCLUSIONS. The authors consistently observed that higher PC was correlated with more aggressive clinicopathologic characteristics. PC was an independent unfavorable prognostic factor. The authors concluded that pericytes should be considered for therapeutic targeting. Cancer 2013. (c) 2012 American Cancer Society.