ALLOGENEIC STIMULATION OF CYTO-TOXIC T-CELLS BY SUPPORTED PLANAR MEMBRANES
ALLOGENEIC STIMULATION OF CYTO-TOXIC T-CELLS BY SUPPORTED PLANAR MEMBRANES
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DOI：
10.1073/pnas.81.19.6159
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
MCCONNELL, HM
中科院分区：
文献类型：
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作者：
BRIAN, AA;MCCONNELL, HM
含有跨膜蛋白H-2Kk的磷脂囊泡在处理过的玻璃表面上孵育时自发融合形成平面膜。荧光脂质探针的图案光漂白表明，这些平面膜是连续的，脂质是移动的，因为它们是在传统的流体双层或单层。这些平面膜中的H-2Kk分子是固定的。这些膜刺激细胞毒性T淋巴细胞与免疫脾细胞培养时。在平面膜的H-2Kk的反应大大增强了从刀豆球蛋白A刺激的脾细胞的上清液，表明相对较少的抗原加工或辅助细胞的介绍发生。发现由纯化的同种异体抗原诱导的细胞毒性T细胞与来自常规混合淋巴细胞培养物的效应物一样对抗体阻断敏感，其中抗体针对被认为通过独立于主要组织相容性抗原的相互作用来加强靶细胞粘附的T细胞表面抗原。
Phospholipid vesicles containing the transmembrane protein H-2Kk spontaneously fuse to form planar membranes when incubated on treated glass surfaces. Pattern photobleaching of fluorescent lipid probes indicates that these planar membranes are continuous and that the lipids are as mobile as they are in conventional fluid bilayers or monolayers. H-2Kk molecules in these planar membranes are immobile. These membranes stimulate cytotoxic T lymphocytes when cultured with immune spleen cells. The response to H-2Kk in planar membranes is greatly enhanced by the addition of supernatant from concanavalin A-stimulated spleen cells, indicating that relatively little antigen processing or presentation by accessory cells occurs. Cytotoxic T cells induced by purified alloantigen are found to be as susceptible to antibody blockade as are effectors from conventional mixed lymphocyte culture, where the antibody is directed against a T cell surface antigen reputed to strengthen target cell adhesion through an interaction independent of major histocompatibility antigens.