Isolated identified Aplysia neurons in cell culture
Isolated identified Aplysia neurons in cell culture
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细胞培养物中分离鉴定的海兔神经元
DOI：
10.1523/jneurosci.01-07-00736.1981
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
I. Levitan
中科院分区：
文献类型：
--
作者：
D. Dagan;I. Levitan
已经开发了用于大的经鉴定的神经元的原代培养的方法。用中性蛋白酶软化神经节的结缔组织鞘。通过钨针操作或用细尼龙丝将其轴突打结来分离单个神经元，并将其固定在鸡血浆凝块或甲基纤维素溶液中。直径达300微米的胞体在培养中几个小时内延伸出长的突起。一个神经元可以产生多达10个突起，这些突起可以以不同的速度生长。细胞内记录显示，培养长达6周的神经元自发和诱发动作电位。培养的神经元对之间形成电突触。在几个培养皿含有神经元从颊神经节，电耦合之间观察到90%的细胞对测试。这种原代培养系统目前正被用来比较的电和生物化学特性的神经元的过程与细胞体，并研究过程再生和突触形成分离的识别神经元之间的必要条件。
Methods have been developed for primary culture of large identified Aplysia neurons. Aplysia ganglia were treated with neutral protease to soften the connective tissue sheath. Individual neurons were isolated either by manipulation with tungsten needles or by tying off their axons with fine nylon filament and were immobilized in a chick plasma clot or a solution of methylcellulose. Somata up to approximately 300 micrometers in diameter extended long processes within several hours in culture. A single neuron produced as many as 10 processes which could grow at different rates. Intracellular recordings showed spontaneous and evoked action potentials in neurons cultured for up to 6 weeks. Electrical synapses formed between pairs of neurons in culture. In several culture dishes containing neurons from buccal ganglia, electrical coupling was observed between 90% of the cell pairs tested. This primary culture system currently is being used to compare the electrical and biochemical properties of neuronal processes with those of cell bodies and to study the conditions necessary for process regeneration and synapse formation between isolated identified neurons.