Molecular Cloning: A Laboratory Manual
Molecular Cloning: A Laboratory Manual
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发表时间：
2001-01
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通讯作者：
J. Sambrook;E. Fritsch;T. Maniatis
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作者：
J. Sambrook;E. Fritsch;T. Maniatis
30年来，分子克隆一直是世界各地实验室技术专长的基础。没有任何一本手册如此受欢迎，或如此有影响力。分子克隆，第四版，由著名的创始作者Joe Sambrook和新的合著者，杰出的HHMI研究者Michael Green，保留了先前版本的高度赞扬的细节和清晰度，并包括由耶鲁大学，马萨诸塞大学，洛克菲勒大学，德克萨斯理工大学，冷泉港实验室，华盛顿大学和其他领先机构的专家从业者委托编写的书的特定章节和协议。技术的理论和历史基础是整个演讲的突出特点，这些信息有助于解决实验问题。对于这个经典作品的第四版，内容已经完全改写，以包括核酸为基础的方法选择为最广泛使用和有价值的分子和细胞生物学实验室。核心章节从第三版已修订，以特色当前的策略和方法，以准备和克隆核酸，基因转移和表达分析。它们增加了12个新的章节，展示了DNA， RNA和蛋白质应该如何制备，评估和操作，以及如何处理数据生成和分析。新内容包括研究细胞组分之间相互作用的方法，如微阵列，下一代测序技术，RNA干扰，以及使用DNA甲基化技术和染色质免疫沉淀的表观遗传分析。为了理解这些技术产生的丰富数据，生物信息学章节描述了分析工具的使用，用于比较基因和蛋白质的序列，并确定基因组之间的共同表达模式。建立在三十年的信任，可靠性和权威，第四版摩尔
Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential. Molecular Cloning, Fourth Edition, by the celebrated founding author Joe Sambrook and new co-author, the distinguished HHMI investigator Michael Green, preserves the highly praised detail and clarity of previous editions and includes specific chapters and protocols commissioned for the book from expert practitioners at Yale, U Mass, Rockefeller University, Texas Tech, Cold Spring Harbor Laboratory, Washington University, and other leading institutions. The theoretical and historical underpinnings of techniques are prominent features of the presentation throughout, information that does much to help trouble-shoot experimental problems. For the fourth edition of this classic work, the content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories. Core chapters from the third edition have been revised to feature current strategies and approaches to the preparation and cloning of nucleic acids, gene transfer, and expression analysis. They are augmented by 12 new chapters which show how DNA, RNA, and proteins should be prepared, evaluated, and manipulated, and how data generation and analysis can be handled. The new content includes methods for studying interactions between cellular components, such as microarrays, next-generation sequencing technologies, RNA interference, and epigenetic analysis using DNA methylation techniques and chromatin immunoprecipitation. To make sense of the wealth of data produced by these techniques, a bioinformatics chapter describes the use of analytical tools for comparing sequences of genes and proteins and identifying common expression patterns among sets of genes. Building on thirty years of trust, reliability, and authority, the fourth edition of Mol