MINIMUM ENERGY ANALYSIS OF MEMBRANE DEFORMATION APPLIED TO PIPET ASPIRATION AND SURFACE-ADHESION OF RED-BLOOD-CELLS
MINIMUM ENERGY ANALYSIS OF MEMBRANE DEFORMATION APPLIED TO PIPET ASPIRATION AND SURFACE-ADHESION OF RED-BLOOD-CELLS
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DOI：
10.1016/s0006-3495(80)85093-4
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发表时间：
1980-01-01
影响因子：
3.4
通讯作者：
EVANS, EA
中科院分区：
文献类型：
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作者：
EVANS, EA
一个实验程序证明，它可以用来确定红细胞膜粘附和膜弹性性能的界面自由能密度。该实验涉及用微量移液管吸取松弛的红细胞，并在发生粘附的表面附近操纵细胞。一个最小自由能的方法来模拟无支撑的膜区域的平衡轮廓，并评估偏导数的总自由能，这对应于微量吸管吸力和粘附的界面自由能密度。红细胞膜的弯曲弹性对抽吸松弛红细胞所需的压力没有显着影响。基于实验证据，红细胞膜的弯曲或曲率弹性模量的上限为10-12尔格（达因-厘米）。粘附实验的分析表明，红细胞粘附的界面自由能密度可以从10-4尔格/cm 2的下限到5-10尔格/cm 2的裂解膜张力建立的上限进行测量。
An experimental procedure is demonstrated which can be used to determine the interfacial free energy density for red cell membrane adhesion and membrane elastic properties. The experiment involves micropipet aspiration of a flaccid red blood cell and manipulation of the cell proximal to a surface where adhesion occurs. A minimum free energy method is developed to model the equilibrium contour of unsupported membrane regions and to evaluate the partial derivatives of the total free energy, which correspond to the micropipet suction force and the interfacial free energy density of adhesion. The bending elasticity of the red cell membrane does not contribute significantly to the pressure required to aspirate a flaccid red cell. Based on experimental evidence, the upper bound for the bending or curvature elastic modulus of the red cell membrane is 10-12 ergs (dyn-cm). Analysis of the adhesion experiment shows that interfacial free energy densities for red cell adhesion can be measured from a lower limit of 10-4 ergs/cm2 to an upper limit established by the membrane tension for lysis of 5-10 ergs/cm2.