DIFFERENTIAL DISTRIBUTION AND MODULATION OF EXPRESSION OF ALPHA1/BETA1 INTEGRIN ON HUMAN ENDOTHELIAL-CELLS
DIFFERENTIAL DISTRIBUTION AND MODULATION OF EXPRESSION OF ALPHA1/BETA1 INTEGRIN ON HUMAN ENDOTHELIAL-CELLS
复制标题
DOI：
10.1083/jcb.114.4.855
复制
发表时间：
1991-08-01
影响因子：
7.8
通讯作者：
TARONE, G
中科院分区：
文献类型：
--
作者：
DEFILIPPI, P;VANHINSBERGH, V;TARONE, G
在本文中，我们报道了整合素复合物α1/β1（一种层粘连蛋白/胶原蛋白受体）在培养的包皮微血管内皮细胞上表达，但在主动脉、脐静脉和股静脉等大血管的内皮细胞上不表达。通过人体组织切片的免疫组织化学染色，在体内也证明了整合素α1/β1在微血管内皮上的限制性表达。 Alpha1特异性抗体与多种组织中的小血管和毛细血管的内皮细胞发生强烈反应，但与脐带静脉和动脉的内皮细胞不发生强烈反应。通过用5ng/ml肿瘤坏死因子α(TNFα)处理，可以在培养的脐静脉内皮细胞中诱导整合素α1的表达。用10(-5)M视黄酸或10nM PMA处理脐静脉内皮后也可诱导α1亚基表达；治疗 48 小时后，α1 整合素的诱导达到最大，并且 TNFα 和 PMA 的共刺激产生了协同效应。 α1整合素的诱导改变了脐静脉内皮细胞的粘附特性，增加了对胶原、层粘连蛋白和层粘连蛋白片段P1的粘附，而对纤连蛋白和层粘连蛋白片段E8的粘附保持恒定。因此，α1 整合素是特定内皮细胞群的标记，其表达赋予与底层基底膜相互作用的独特特性。
In this paper we report that the integrin complex alphal/beta1, a laminin/collagen receptor, is expressed on cultured foreskin microvascular endothelium, but is absent on endothelial cells from large vessels such as the aorta and umbilical and femoral veins. The restricted expression of integrin alpha1/beta1 to microvascular endothelium was also demonstrated in vivo, by immunohistochemical staining of human tissue sections. Alpha1 specific antibodies reacted strongly with endothelial cells of small blood vessels and capillaries in several tissues, but not with endothelium of vein and arteries of umbilical cord. Expression of integrin alphal can be induced in cultured umbilical vein endothelial cells by treatment with 5 ng/ml tumor necrosis factor alpha (TNFalpha). Induction of alpha1 subunit expression also occurred after treatment of umbilical vein endothelium with 10(-5) M retinoic acid or with 10 nM PMA; Maximal induction of alpha1 integrin was reached after 48 h of treatment and costimulation with TNFalpha and PMA resulted in a synergistic effect. The induction of alpha1 integrin changed the adhesive properties of umbilical vein endothelial cells, by increasing the adhesiveness to collagen, laminin, and laminin fragment P1, while adhesion to fibronectin and laminin fragment E8 remained constant. The alpha1 integrin is thus a marker of a specific population of endothelial cells and its expression confers distinctive properties of interaction with the underlying basal membrane.