An established immune response against ovalbumin is suppressed by a transferable serum factor produced after ovalbumin feeding:: A role of CD25+ regulatory cells
An established immune response against ovalbumin is suppressed by a transferable serum factor produced after ovalbumin feeding:: A role of CD25+ regulatory cells
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DOI：
10.1046/j.1365-3083.2002.t01-1-01079.x
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发表时间：
2002-05-01
影响因子：
3.7
通讯作者：
Dahlgren, UI
中科院分区：
文献类型：
--
作者：
Karlsson, MR;Kahu, H;Dahlgren, UI
我们以前已经证明，喂食卵清蛋白(OVA)的大鼠在血清中产生耐受活性，这种活性在转移时诱导对OVA的耐受，并抑制对旁观者抗原的免疫反应。在这里，我们扩展了这些研究，并分析了血清中的耐受活性是否可以抑制受者已建立的免疫反应。OVA组大鼠在血清移植前4周和1周分别用OVA免疫，OVA组大鼠血清移植后1周抗OVA迟发型超敏反应(DTH)显著低于对照组，血清移植后2周和4周免疫球蛋白(Ig)抗OVA抗体水平显著低于对照组。与血清转移的OVA相对应的单体OVA不会引起DTH反应或抗OVA抗体水平的降低。在体外，来自耐受血清接受者的OVA刺激的脾细胞的增殖明显低于来自对照组的脾细胞。这种体外抑制似乎是由CD25(+)细胞群介导的，因为从OVA刺激的脾细胞悬液中去除CD25(+)细胞会导致接受耐受血清的大鼠培养的细胞增殖增加。我们的结果表明，耐受性血清因子可以抑制受体动物已建立的免疫反应，可能是通过诱导调节性CD25(+)细胞。这种能力是否可能被用来影响慢性炎症状况，还需要进行调查。
We have previously demonstrated that rats fed ovalbumin (OVA) develop a tolerogenic activity in serum, which upon transfer induces tolerance to OVA and suppression of the immune response to a bystander antigen. Here, we have extended these studies and analysed if the tolerogenic activity in serum could suppress an established immune response in the recipients. Rats were immunized with OVA, 4 and 1 week prior to the transfer of serum from either OVA-fed or control animals.Rats that received serum from OVA-fed donors had significantly lower delayed-type hypersensitivity (DTH) reaction against OVA 1 week after the serum transfer compared with the controls, and the levels of immunoglobulin (IgG) anti-OVA antibodies were significantly lower 2 and 4 weeks after serum transfer. Monomeric OVA in amounts corresponding to the OVA transferred with serum did not induce the reduction of DTH response or IgG anti-OVA antibody levels. In vitro, the proliferation of OVA-stimulated spleen cells, taken from recipients of tolerogenic serum, was significantly lower compared with spleen cells from the controls. The in vitro suppression seemed to be mediated by a population of CD25(+) cells, because the removal of such cells from OVA-stimulated spleen cell suspensions resulted in increased proliferation in cultures from rats receiving tolerogenic serum. Our results showed that the tolerogenic serum factor can suppress an established immune response in recipient animals, possibly through induction of regulatory CD25(+) cells. Whether this capacity might be used to influence chronic inflammatory conditions needs to be investigated.