THE CLASS-B SCAVENGER RECEPTORS SR-BI AND CD36 ARE RECEPTORS FOR ANIONIC PHOSPHOLIPIDS
THE CLASS-B SCAVENGER RECEPTORS SR-BI AND CD36 ARE RECEPTORS FOR ANIONIC PHOSPHOLIPIDS
复制标题
DOI：
10.1074/jbc.270.27.16221
复制
发表时间：
1995-07-07
影响因子：
4.8
通讯作者：
KRIEGER, M
中科院分区：
文献类型：
--
作者：
RIGOTTI, A;ACTON, SL;KRIEGER, M
细胞表面受体对细胞膜外叶中的阴离子磷脂和脂蛋白的特异性识别可能在多种生理和病理生理过程中发挥重要作用（例如，网状内皮系统或脂蛋白稳态对受损或衰老细胞的识别）。几个研究者已经描述了阴离子磷脂与细胞的结合，并且最近报道了磷脂酰丝氨酸（PS）与部分纯化的类似于95-kDa膜蛋白的结合（Sambrano，G. R.，和Steinberg，D.等人（1995）Proc. Acad. Sci.联合S. A. 92，1396-1400）。在转染细胞中使用直接结合和配体竞争试验，我们发现两种B类清道夫受体SR-BI和CD 36可以紧密结合PS和含磷脂酰肌醇（PI）的脂质体（PS脂质体与SR-BI结合的Kd类似于15 μ g磷脂/ml或0.18 nM（mol PS脂质体/l）），但不能结合磷脂酰胆碱、磷脂酰乙醇胺或鞘磷脂脂质体。PS和PI脂质体，但不是其他人，可以有效地与PS脂质体和修饰或天然脂蛋白结合这些受体。磷脂酸，另一种阴离子磷脂，也可以竞争，但不如PS或PI有效。B类清道夫受体是第一个分子上明确定义的、特异性的阴离子磷脂细胞表面受体。
The specific recognition of anionic phospholipids in the outer leaflets of cell membranes and lipoproteins by cell surface receptors may play an important role in a variety of physiologic and pathophysiologic processes (e.g. recognition of damaged or senescent cells by the reticuloendothelial system or lipoprotein homeostasis). Several investigators have described anionic phospholipid binding to cells, and phosphatidylserine (PS) binding to a partially purified similar to 95-kDa membrane protein has recently been reported (Sambrano, G. R., and Steinberg, D. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 1396-1400). Using both direct binding and ligand competition assays in transfected cells, we have found that two class B scavenger receptors, SR-BI and CD36, can tightly bind PS and phosphatidylinositol (PI)-containing liposomes (K-d for PS liposome binding to SR-BI is similar to 15 mu g phospholipid/ml or 0.18 nM (mol PS liposomes/l)), but not phosphatidylcholine, phosphatidylethanolamine, or sphingomyelin liposomes. PS and PI liposomes, but not the others, could effectively compete with PS liposomes and modified or native lipoproteins for binding to these receptors. Phosphatidic acid, another anionic phospholipid, could also compete, but was not as effective as PS or PI. Class B scavenger receptors are the first molecularly well-defined, specific cell surface receptors for anionic phospholipids to be described.