In vivo cartilage strain increases following medial meniscal tear and correlates with synovial fluid matrix metalloproteinase activity.
In vivo cartilage strain increases following medial meniscal tear and correlates with synovial fluid matrix metalloproteinase activity.
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DOI：
10.1016/j.jbiomech.2015.02.030
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发表时间：
2015-06-01
影响因子：
2.4
通讯作者：
DeFrate LE
中科院分区：
文献类型：
--
作者：
Carter TE;Taylor KA;Spritzer CE;Utturkar GM;Taylor DC;Moorman CT 3rd;Garrett WE;Guilak F;McNulty AL;DeFrate LE
半月板撕裂是常见的损伤，而部分半月板切除术是一种常见的治疗选择，一般半月板损失是骨关节炎发展的风险因素。本研究的目的是测量半月板撕裂患者的体内胫股软骨接触模式与这些关节滑液中软骨卡他汀生物标志物的关系。磁共振成像和双平面荧光镜检查相结合，用于确定膝关节的体内运动和软骨接触力学。在进行准静态弓步时，分析了孤立性内侧半月板撕裂的受试者，并将对侧未受伤的膝关节作为对照。从受伤的膝关节收集滑液，并测量基质金属蛋白酶（MMP）活性、硫酸化糖胺聚糖、软骨寡聚基质蛋白、前列腺素E2和II型胶原裂解生物标志物C2C。与对侧对照膝关节相比，受伤膝关节内侧室的接触应变显着增加。在外侧间室中，损伤膝关节的接触应变仅在最大屈曲角（105°）时显著增加。最大屈曲时的平均软骨应变与滑液中的总MMP活性呈正相关。这些发现表明，关节损伤导致正常关节功能丧失和关节软骨应变增加，这与滑液中总MMP活性升高相关。增加的应变和总MMP活性可能反映或潜在地促成骨关节炎的早期发展，骨关节炎是在骨关节损伤后观察到的。
Meniscal tears are common injuries, and while partial meniscectomy is a frequent treatment option, general meniscus loss is a risk factor for the development of osteoarthritis. The goal of this study was to measure the in vivo tibiofemoral cartilage contact patterns in patients with meniscus tears in relation to biomarkers of cartilage catabolism in the synovial fluid of these joints. A combination of magnetic resonance imaging and biplanar fluoroscopy was used to determine the in vivo motion and cartilage contact mechanics of the knee. Subjects with isolated medial meniscus tears were analyzed while performing a quasi-static lunge, and the contralateral uninjured knee was used as a control. Synovial fluid was collected from the injured knee and matrix metalloproteinase (MMP) activity, sulfated glycosaminoglycan, cartilage oligomeric matrix protein, prostaglandin E2, and the collagen type II cleavage biomarker C2C were measured. Contact strain in the medial compartment increased significantly in the injured knees compared to contralateral control knees. In the lateral compartment, the contact strain in the injured knee was significantly increased only at the maximum flexion angle (105°). The average cartilage strain at maximum flexion positively correlated with total MMP activity in the synovial fluid. These findings show that meniscal injury leads to loss of normal joint function and increased strain of the articular cartilage, which correlated to elevated total MMP activity in the synovial fluid. The increased strain and total MMP activity may reflect, or potentially contribute to, the early development of osteoarthritis that is observed following meniscal injury.