Assessment of 1p/1.9q deletions by fluorescence in situ hybridization in gliomas
Assessment of 1p/1.9q deletions by fluorescence in situ hybridization in gliomas
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DOI：
10.1016/j.cancergencyto.2008.03.009
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发表时间：
2008-07-15
影响因子：
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通讯作者：
Reddy, Kavita S.
中科院分区：
文献类型：
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作者：
Reddy, Kavita S.
本研究对77例石蜡包埋组织样本进行了FISH检测胶质瘤中1p/19q缺失的效果评估，其中42例（55%）1p/19q编码缺失阳性；42人中有7人有神经胶质瘤病史。在一个案例中，分析失败了。其余34例为阴性；其中3人有神经胶质瘤病史，1人有相反的1q/19p缺失。10例复发性胶质瘤中大多数已进展，70%有1p/19q缺失。在大多数1p/19q编码情况下，两个探针组的信号模式都是单个红色标记信号和两个绿色参考信号（1R2G）。一个病例在染色体1和19 （1R2G和2R4G）上有不同的信号模式，如在多体细胞中所见。如在四倍体细胞中所见，三例有两个靶信号和四个控制信号（2R4G）。在二倍体和多倍体细胞中，有11个细胞具有复杂的信号模式（1R2G/< nRnG）。这些复杂病例中的大多数（11例中有8例，即73%）为高级别少突胶质细胞瘤（n = 4）或少突星形细胞瘤（n = 4）。少突胶质细胞瘤（n = 40）、少星形细胞瘤（n = 21）和星形细胞瘤（n = 8）中1p/19q联合缺失的频率分别为65%、60%和25%。本文阐明了在胶质瘤中观察到的各种FISH信号模式，以及为提高诊断需要统一的命名法。(C) 2008爱思唯尔公司版权所有。
This study assessed the efficacy of FISH for detecting 1p/19q deletion in gliomas on 77 paraffin-embedded tissue samples, of which 42 cases (55%) were positive for 1p/19q codeletion; 7 of the 42 had a previous history of glioma. In one case, analysis failed. The remaining 34 cases were negative; of these, three had a previous history of glioma and one had the reverse 1q/19p deletion. A majority of the 10 recurrent gliomas had progressed, and 70% had a 1p/19q deletion. The signal pattern in a majority of 1p/19q codeletion cases was a single red marker signal and two green reference signals (1R2G) for both probe sets. One case had a different signal pattern for chromosomes I and 19 (1R2G and 2R4G), as seen in polysomy cells. Three cases had two target and four control signals (2R4G), as seen in tetraploid cells. Eleven had complex signal patterns seen in diploid and polyploid cells (1R2G/< nRnG). A majority of these complex cases (8 of 11, or 73%) had high-grade oligodendroglioma (n = 4) or oligoastrocytoma (n = 4). The frequency of 1p/19q combined deletion in oligodendroglioma (n = 40), oligoastrocytoma (n = 21), and astrocytoma (n = 8) was 65, 60, and 25%, respectively. Elucidated in this paper are the various FISH signal patterns observed in gliomas and a need for a uniform nomenclature for improved diagnosis. (C) 2008 Elsevier Inc. All rights reserved.