Is reliable in vivo detection of stem cell viability possible in a large animal model of myocardial injury?
Is reliable in vivo detection of stem cell viability possible in a large animal model of myocardial injury?
复制标题
在大型动物心肌损伤模型中是否可以可靠地体内检测干细胞活力？
DOI：
10.1161/circulationaha.112.119305
复制
发表时间：
2012
期刊：
影响因子：
37.8
通讯作者：
Yang,PhillipC
中科院分区：
文献类型：
--
作者：
Yang,PhillipC
干细胞的体内细胞和分子成像已经被开发出来，以在一个完整的、活的有机体中在最基本的水平上表征生物过程。随着衰竭心脏的细胞治疗的出现，评估移植的干细胞在体内的植入和存活的能力是衡量治疗效果的关键指标。干细胞至少必须存活下来，才能恢复受损的心肌。1、2细胞存活信号具有生理学相关性，因为它可能与所产生的心肌修复相关。心脏干细胞活性的活体成像有两个主要考虑因素：(1)分子和细胞信号的放大；(2)高空间和时间分辨率的心肌成像。人们普遍认为，没有一种单一的成像方式可以满足体内干细胞成像的所有需求。然而，优化技术目标的成像模式可能会提取关于移植细胞的细胞和分子事件的有意义的信息。在临床前模型中，评估体内干细胞存活的主要成像手段包括放射性核素成像、光学成像和核磁共振成像。这些模式通常用于小动物模型。然而，体内细胞和分子成像技术从小动物模型直接转移到大动物模型并不是一帆风顺的。
In vivo cellular and molecular imaging of the stem cells has been developed to characterize the biological processes at the most fundamental level in an intact, living organism. With the emergence of cell-based therapy in a failing heart, the capability to evaluate engraftment and survival of the transplanted stem cells in vivo represents a critical measure of therapeutic efficacy. The stem cells at the very least must survive to restore the injured myocardium. 1, 2 Cell viability signal holds physiological relevance because it may correlate with the resultant myocardial restoration. There are 2 primary considerations for in vivo imaging of stem cell viability in the heart:(1) amplification of molecular and cellular signals and (2) high spatial and temporal resolution imaging of the myocardium. It is widely acknowledged that there is no single imaging modality that will fulfill all needs of in vivo stem cell imaging. However, an imaging modality that optimizes the technical objectives may extract meaningful information on cellular and molecular events of the transplanted cells. The predominant imaging modalities to assess stem cell survival in vivo in preclinical models consist of radionuclide imaging, optical imaging, and MRI. These modalities are commonly used in small animal models. Direct transfer of in vivo cellular and molecular imaging techniques from small to large animal models, however, has not been straightforward.