ELECTRON MICROSCOPE STUDIES ON ULTRATHIN SECTIONS OF MUSCLE
ELECTRON MICROSCOPE STUDIES ON ULTRATHIN SECTIONS OF MUSCLE
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DOI：
10.1084/jem.99.2.201
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发表时间：
1954-01-01
影响因子：
15.3
通讯作者：
SPIRO, D
中科院分区：
文献类型：
--
作者：
HODGE, AJ;HUXLEY, HE;SPIRO, D
本文对青蛙（缝匠肌）、家兔（腰肌）、大鼠（心肌）、苍蝇（飞行肌）的横纹肌和蛤（内收肌）的平滑肌作了电镜观察。给出了切片厚度为200-400埃，分辨率为30-40埃。在蝇飞行肌中，肌丝呈明显分离的形态。在青蛙和兔子的肌肉中，它们在正常情况下是如此紧密地堆积在一起，以至于它们作为单独单位的身份几乎消失了。在所有类型的横纹肌检查，细丝内的肌原纤维排列在一个连续的和高度规则的六边形阵列。直径。这里看到的细丝的直径小于在干燥的阴影材料中观察到的直径，但是细丝之间存在明显量的材料。在青蛙和兔骨骼肌和大鼠心肌中观察到的400 A轴向周期似乎延伸穿过细丝之间的间质材料。这些结果的意义进行了讨论。
Thin sections of striated muscle from frog (sartorius), rabbit (psoas) rat (heart muscle) fly (flight muscle) and of smooth muscle from clam (adductor) were examined in the electron microscope; 10 illus. are presented. The sections were 200-400 A in thickness and permitted a resolution of 30-40 A. In fly flight muscle, the myofilbrils appeared as distinct and well-separated morph. units, but in frog and rabbit muscle they were so closely packed under normal conditions that their identity as separate units was almost lost. In all types of striated muscle examined, the filaments were arranged within the myofibrils in a continuous and highly regular hexagonal array. The diam. of the filaments seen here was smaller than that observed in dried, shadowed material, but an appreciable quantity of material was present between the filaments. The 400 A axial period seen in frog and rabbit skeletal muscle and in rat heart muscle appeared to extend across the interstitial material between the filaments. The significance of these results is discussed.