Localized recombination drives diversification of killing spectra for phage-derived syringacins
Localized recombination drives diversification of killing spectra for phage-derived syringacins
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DOI：
10.1038/s41396-018-0261-3
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发表时间：
2019-02-01
期刊：
影响因子：
11
通讯作者：
Hockett, Kevin L.
中科院分区：
文献类型：
--
作者：
Baltrus, David A.;Clark, Meara;Hockett, Kevin L.
为了更好地了解相同细菌物种的成员之间的拮抗相互作用的潜力，我们已经调查了细菌素杀死活性在不同的植物病原体假单胞菌的菌株套件。我们的数据表明，从噬菌体衍生的细菌素的P.lingae（R型lingacins）的杀伤活性是广泛的。尽管细菌素活性的总体多样性很高，但菌株可以大致分为五种主要的杀灭类型和两种主要的敏感性类型。此外，我们发现，杀死活性开关之间的菌株和开关与本地重组的两个基因，共同编码的蛋白质，指定细菌素靶向。最后，我们表明，噬菌体衍生的细菌素的杀伤活性可以交换菌株之间简单地通过表达这两个基因的反式。总体而言，我们的研究的特点广泛多样性的杀伤活性的噬菌体衍生的细菌素的P. pastingae跨菌株，并强调本地化重组的力量，改变表型，介导菌株的相互作用在自然种群和社区的演变。
To better understand the potential for antagonistic interactions between members of the same bacterial species, we have surveyed bacteriocin killing activity across a diverse suite of strains of the phytopathogen Pseudomonas syringae. Our data demonstrate that killing activity from phage-derived bacteriocins of P. syringae (R-type syringacins) is widespread. Despite a high overall diversity of bacteriocin activity, strains can broadly be classified into five main killing types and two main sensitivity types. Furthermore, we show that killing activity switches frequently between strains and that switches correlate with localized recombination of two genes that together encode the proteins that specify bacteriocin targeting. Lastly, we demonstrate that phage-derived bacteriocin killing activity can be swapped between strains simply through expression of these two genes in trans. Overall, our study characterizes extensive diversity of killing activity for phage-derived bacteriocins of P. syringae across strains and highlights the power of localized recombination to alter phenotypes that mediate strain interactions during evolution of natural populations and communities.