Changes in prostaglandin secretion by the regressing bovine corpus luteum
Changes in prostaglandin secretion by the regressing bovine corpus luteum
复制标题
DOI：
10.1016/s0090-6980(02)00148-x
复制
发表时间：
2003-01-01
影响因子：
2.9
通讯作者：
Miyamoto, A
中科院分区：
文献类型：
--
作者：
Hayashi, K;Acosta, TJ;Miyamoto, A
对奶牛退化黄体(CL)分泌前列腺素(PGs)进行了研究。6头奶牛在第8-9天(第0天=发情)将微透析系统(MDS)的微毛细管透析膜植入CL，肌肉注射前列腺素(PG)F-2α类似物(Estrumate)。以诱导黄体溶解。卵巢静脉血浆中前列腺素F(2α)和前列腺素E(2α)浓度在实验期间比颈静脉血浆浓度高4~13倍(P<0.001)，从用药后24小时开始升高(P<0.05)。环氧合酶-2(COX-2mRNA)在用药后2 h和24 h表达增加(P<0.05)。结果表明，PGF(2α)和PGE(2α)的局部释放和COX-2mRNA的表达均有不同程度的变化。在黄体溶解的后期，在消退的CL中，Estrumate增加了。因此，前列腺素的直线分泌可能参与了结构性黄体溶解而不是功能性黄体溶解的局部机制。(C)2002 Elsevier Science Inc.保留所有权利。
Secretion of prostaglandins (PGs) by the regressing corpus luteum (CL) was investigated in the cow. Six cows were implanted with microcapillary dialysis membranes of a microdialysis system (MDS) into the CL during Days 8-9 (Day 0 = estrus), and a prostaglandin (PG) F-2alpha analogue (Estrumate) was injected intramuscularly (i.m.) to induce luteolysis. Acute increases in intraluteal release of PGF(2alpha) and PGE(2) were observed during the first 4h, followed by decreases over the next 9 h. Intraluteal release of both PGs gradually increased again during the period 48-72 h. Concentrations of PGF(2alpha) in ovarian venous plasma (OVP) were 4-13 times higher than those of jugular venous plasma (JVP) (P < 0.001) during the period of the experiment, and increased from 24 h after treatment with Estrumate (P < 0.05). Cyclooxygenase (COX)-2 mRNA expression increased (P < 0.05) at 2 and 24 h after treatment with Estrumate. The results indicated that local release of PGF(2 alpha) and PGE(2 alpha) and COX-2 mRNA expression were. increased by Estrumate in the regressing CL at the later stages of luteolysis. Thus, lineal secretion of PGs may be involved in the local mechanism for structural rather than functional luteolysis. (c) 2002 Elsevier Science Inc. All rights reserved.