Detection of reovirus RNA in hepatobiliary tissues from patients with extrahepatic biliary atresia and choledochal cysts
Detection of reovirus RNA in hepatobiliary tissues from patients with extrahepatic biliary atresia and choledochal cysts
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DOI：
10.1002/hep.510270603
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发表时间：
1998-06-01
期刊：
影响因子：
13.5
通讯作者：
Brown, WR
中科院分区：
文献类型：
--
作者：
Tyler, KL;Sokol, RJ;Brown, WR
肝外胆道闭锁和胆总管囊肿是引起小儿梗阻性黄疸的重要原因。一般来说，病毒，特别是呼肠孤病毒，长期以来一直被认为是可能的病原体，负责煽动导致这些婴儿阻塞性胆管病的炎症过程。为了确定呼肠孤病毒感染是否与这些疾病相关，我们使用了一种敏感和特异的逆转录聚合酶链反应（RT-PCR），旨在从肝脏和/或胆道组织提取物中扩增呼肠孤病毒L1基因片段的一部分的技术。这些组织是在肝活检或外科手术时从23例EHBA患者、9例CDC患者和33例其他肝胆疾病患者中获得的。还分析了17例无已知肝脏或胆道疾病死亡的患者尸检时获得的肝脏和胆道组织。55%的EHBA患者和78%的CDC患者的肝和/或胆组织中检测到呼肠孤病毒RNA，21%的其他肝胆疾病患者和12%的尸检病例的肝和/或胆组织提取物中也发现呼肠孤病毒RNA。EHBA和CDC患者组织中呼肠孤病毒RNA的患病率显著高于其他肝胆疾病患者（χ 2，P = 0.012 EHBA vs. OTHER，P = 0.001 CDC vs. OTHER）或尸检病例（χ 2，P = 0.006 EHBA vs. AUTOPSY，P <0.001 CDC vs. AUTOPSY）。
Extrahepatic biliary atresia (EHBA) and choledochal cysts (CDC) are important causes of obstructive jaundice in pediatric patients. Viruses in general, and reoviruses in particular, have long been considered as possible etiologic agents responsible for inciting the inflammatory process that leads to these infantile obstructive cholangiopathies, In an effort to determine whether reovirus infection is associated with these disorders, we used a sensitive and specific reverse-transcriptase polymerase chain reaction (RT-PCR) technique designed to amplify a portion of the reovirus L1 gene segment from extracts of liver and/or biliary tissues. These tissues were obtained at the time of liver biopsy or surgical procedures from 23 patients with EHBA, 9 patients with CDC, and 33 patients with other hepatobiliary diseases. Hepatic and biliary tissues obtained at autopsy from 17 patients who died without known liver or biliary disease were also analyzed. Reovirus RNA was detected in hepatic and/or biliary tissues from 55% of patients with EHBA and 78% of patients with CDC, Reovirus RNA was found also in extracts of hepatic and/or biliary tissue from 21% of patients with other hepatobiliary diseases and in 12% of autopsy cases. The prevalence of reovirus RNA in tissues from patients with EHBA and CDC was significantly greater than that in patients with other hepatobiliary diseases (chi(2) P = .012 EHBA vs. OTHER, P = .001 CDC vs. OTHER), or AUTOPSY cases (chi(2) P = .006 EHBA vs, AUTOPSY, P < .001 CDC vs. AUTOPSY).