The Use of Intact Cellular Activation Systems in Genetic Toxicology Assays
The Use of Intact Cellular Activation Systems in Genetic Toxicology Assays
复制标题
完整细胞激活系统在遗传毒理学测定中的应用
DOI：
10.1007/978-1-4613-3694-5_2
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
L. Oglesby
中科院分区：
文献类型：
--
作者：
R. Langenbach;L. Oglesby
Most of the target cells used in in vitro genetic toxicology assay systems have limited endogenous xenobiotic metabolic capacity, and most environmental chemicals to which humans are exposed exist in a promutagenic and/or procarcinogenic form, thereby requiring metabolic activation to manifest biological activity.(1,2) In order to use these organisms to detect agents which many be genotoxic, exogenous metabolic activation is usually required to determine biological activity of these chemicals in in vitro systems. To date, most in vitro short-term assay systems have included rat liver homogenates (S-9) for metabolic activation. Several studies, however, indicate that S-9 preparations may not simulate the in vivo activation process and that intact cells may be more representative of in vivo activation conditions.(3−8) In fact, the use of S-9 preparations may yield artifactual results.(6) Therefore, one of the goals of our laboratory has been the development and use of intact cellular activation systems in assays to detect genotoxic agents, as well as to study fundamental mechanisms associated with the bioactivation process.
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