Insertion of sequences into the 3' untranslated region of a replication-competent spleen necrosis virus vector disrupts env gene expression.
Insertion of sequences into the 3' untranslated region of a replication-competent spleen necrosis virus vector disrupts env gene expression.
复制标题
将序列插入具有复制能力的脾坏死病毒载体的 3 非翻译区会破坏 env 基因的表达。
DOI：
10.1007/s007050050486
复制
发表时间：
1999
影响因子：
2.7
通讯作者：
Hu,WS
中科院分区：
文献类型：
--
作者：
Yin,PD;Hu,WS
所有具有复制能力的逆转录病毒的基因组都含有调节基因表达的顺式作用元件。然而，其中许多要素的特性仍有待确定。将序列插入到具有复制能力的脾坏死病毒（SNV）载体的3′非翻译区，破坏了其复制能力。序列和大小（0.4-kb至1.6-kb）不同的DNA片段均导致该缺陷。遗传补偿实验和免疫组化结果显示，tenv基因表达缺失。北方分析表明存在正确大小的剪接的病毒mRNA，尽管与野生型载体相比水平降低。很可能是在转录后步骤发生了基因表达的阻断。这些结果表明，插入SNV 3′非翻译区的序列破坏了与组成型转运元件不同的顺式作用元件的功能。
The genomes of all replication-competent retroviruses contain cis-acting elements that regulate gene expression. However, the identities of many of these elements remain to be characterized. Inserting sequences into the 3′ untranslated region of a replication-competent spleen necrosis virus (SNV) vector disrupted its ability to replicate. Inserts varying in sequences and sizes (0.4-kb to 1.6-kb) all resulted in this defect. Genetic compensation experiments and immunostaining revealed thatenvgene expression was deficient. Northern analysis indicated the presence of spliced viral mRNA of the correct size although at a reduced level compared to a wildtype vector. It is likely that the block inenvexpression occurs at a post-transcriptional step. These results suggest that the function of a cis-acting element distinct from the constitutive transport element is disrupted by the inserted sequences into the 3′ untranslated region of SNV.