RATE OF MACROMOLECULAR-SYNTHESIS THROUGH CELL-CYCLE OF YEAST SACCHAROMYCES-CEREVISIAE
RATE OF MACROMOLECULAR-SYNTHESIS THROUGH CELL-CYCLE OF YEAST SACCHAROMYCES-CEREVISIAE
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DOI：
10.1073/pnas.75.9.4384
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发表时间：
1978-01-01
影响因子：
11.1
通讯作者：
MCLAUGHLIN, CS
中科院分区：
文献类型：
--
作者：
ELLIOTT, SG;MCLAUGHLIN, CS
采用离心洗脱的方法，将酿酒酵母的细胞按其在细胞周期中所处的位置进行指数级均衡分离。对大分子合成进行了研究。DNA合成是周期性的，但RNA和蛋白质的合成速度呈指数级增长。双向电泳法被用来确定单个蛋白质的合成速度，从系统中迁移的1000多种蛋白质中挑选出111种更丰富的细胞蛋白质进行分析。所有被检测的蛋白质的合成速度都呈指数级增长。
Centrifugal elutriation was used to separate cells of S. cerevisiae in balanced exponential growth according to position in the cell cycle. Macromolecular synthesis was examined. DNA synthesis was found to be periodic, but RNA and protein synthesis showed an exponential increase in rate. Two-dimensional electrophoresis was used to determine the rate of synthesis of individual proteins, with 111 of the more abundant cellular proteins selected for analysis from among the more than 1000 proteins that migrate in the system. All the examined proteins showed an exponentially increasing rate of synthesis.