In vitro formation of a c-type cytochrome
In vitro formation of a c-type cytochrome
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DOI：
10.1073/pnas.132259099
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发表时间：
2002-06-11
影响因子：
11.1
通讯作者：
Ferguson, SJ
中科院分区：
文献类型：
--
作者：
Daltrop, O;Allen, JWA;Ferguson, SJ
C型细胞色素对于几乎所有生物体都是必需的；它们的特征是血红素通过两个硫醚键与 Cys-Xaa-Xaa-Cys-His 肽基序共价连接到蛋白质上。与30年来的观点相反，我们在此证明，在温和条件下且没有任何生物合成装置的情况下，c型细胞色素可以在体外由血红素和脱辅基蛋白形成。如果避免 Cys-Xaa-Xaa-Cys-His 基序内形成二硫键，则会发生此反应。这对于了解体内细胞色素 c 的组装具有重要意义。
C-type cytochromes are essential for almost all organisms; they are characterized by the covalent attachment of heme to protein through two thioether bonds to a Cys-Xaa-Xaa-Cys-His peptide motif. Here we show, contrary to opinion of 30 years standing, that a c-type cytochrome can form from heme and apoprotein in vitro under mild conditions and in the absence of any biosynthesis apparatus. This reaction occurs provided formation of a disulfide bond within the Cys-Xaa-Xaa-Cys-His motif is avoided. There are important implications for understanding in vivo cytochrome c assembly.