Similar mechanisms might be triggered by alternative external stimuli that induce dormancy release in grape buds
Similar mechanisms might be triggered by alternative external stimuli that induce dormancy release in grape buds
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DOI：
10.1007/s00425-008-0720-6
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发表时间：
2008-06-01
期刊：
影响因子：
4.3
通讯作者：
Or, Etti
中科院分区：
文献类型：
--
作者：
Halaly, Tamar;Pang, Xuequn;Or, Etti
具有相似的表达谱的基因的检测后，应用不同的刺激，触发芽打破可能构成有效的工具，用于识别的途径与休眠释放的核心作用。我们比较了热休克（HS）和氢氰胺（HC）的影响，并证明HS导致更早和更高的萌芽水平。在这两种处理后，诱导过氧化氢酶，乙醇脱氢酶和丙酮酸脱羧酶的转录水平的变化。然而，转录水平的变化的时间和程度不同。HS处理的芽中发生的变化较早，HC处理的芽中发生的变化更强烈。两种治疗后转录水平的变化都是暂时的。HS处理后基因表达的快速和短暂变化与该处理产生的更快和更高水平的萌芽相关。这种相关性可能表明，报道的分子事件是机械参与休眠解除。为了验证这一假设，即暂时的氧化胁迫是诱导休眠解除的机制的一部分，我们分析了HS和HC处理对抗坏血酸过氧化物酶，谷胱甘肽还原酶，硫氧还蛋白h，谷胱甘肽S-转移酶和蔗糖合成酶基因的表达的影响，发现它们是由两种治疗诱导的类似模式。总之，这些发现表明，类似的细胞过程可能是由不同的刺激，导致休眠解除触发，并与假设，即暂时的氧化应激和呼吸应激可能是导致芽断裂的机制的一部分是一致的。
The detection of genes having similar expression profiles following the application of different stimuli that trigger bud break may constitute potent tools for the identification of pathways with a central role in dormancy release. We compared the effects of heat shock (HS) and hydrogen cyanamide (HC) and demonstrated that HS leads to earlier and higher bud-break levels. Changes in transcript levels of catalase, alcohol dehydrogenase and pyruvate decarboxylase were induced following both treatments. However, timing and extent of changes in transcript level differed. Changes occurred earlier in HS-treated buds and were more intense in HC-treated buds. The changes in transcript levels after both treatments were temporary. The rapid and short-lasting changes in gene expression following HS treatment correlated with the faster and higher level of bud-break that this treatment exerted. This correlation may propose that the reported molecular events are mechanistically involved in dormancy release. To test the hypothesis that temporary oxidative stress is part of the mechanism inducing dormancy release, we analyzed the effect of HS and HC treatments on the expression of ascorbate peroxidase, glutathione reductase, thioredoxin h, glutathione S-transferase and sucrose synthase genes and found that they were induced by both treatments in a similar pattern. Taken together, these findings propose that similar cellular processes might be triggered by different stimuli that lead to dormancy release, and are consistent with the hypothesis that temporary oxidative stress and respiratory stress might be part of the mechanism that leads to bud break.