Interactions and DNA transfer between Agrobacterium tumefaciens, the Ti-plasmid and the plant host
Interactions and DNA transfer between Agrobacterium tumefaciens, the Ti-plasmid and the plant host
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根癌农杆菌、Ti 质粒和植物宿主之间的相互作用和 DNA 转移
DOI：
10.1098/rspb.1979.0026
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
R. Villarroel
中科院分区：
文献类型：
--
作者：
J. Schell;M. Montagu;M. D. Beuckeleer;M. Block;A. Depicker;M. Wilde;G. Engler;C. Genetello;J. Hernalsteens;M. Holsters;J. Seurinck;B. Silva;F. Vliet;R. Villarroel
根癌农杆菌是一种革兰氏阴性细菌，具有在双子叶植物中诱导肿瘤转化的独特能力。近年来，这种转化的机制和生物学意义已被阐明。根癌农杆菌菌株含有一个大的染色体外DNA质粒（Ti质粒）。该Ti质粒负责农杆菌菌株的致癌特性。Ti质粒的一个特殊片段，包含决定肿瘤生长模式和所谓的“意见”合成的信息，e. G.章鱼碱（N-α-（D-1-羧乙基）-L-精氨酸）和胭脂碱（N-α-（1，3-二羧丙基）-L-精氨酸）在转化的植物细胞中转移并稳定维持和表达。这一现象可以理解为细菌质粒DNA在植物细胞中的“遗传定殖”，从而转化的植物细胞将产生并分泌到培养基中的氨基酸衍生物（冠菌素），携带Ti质粒的农杆菌可以选择性地使用这些氨基酸衍生物作为碳源和氮源。
Agrobacterium tumefaciens is a gram-negative bacterium with the unique capacity to induce neoplasmic transformations in dicotyledonous plants. Recently, both the mechanism and the biological significance of this transformation have been elucidated. Agrobacterium tumefaciens strains contain a large extrachromosomal DNA plasmid (the Ti-plasmid). This Ti-plasmid is responsible for the oncogenic properties of Agrobacterium strains. A particular segment of the Ti-plasmid, containing information determining the tumorous growth pattern and the synthesis of so-called ‘opines’, e. g. octopine (N-α-(D-l-carboxyethyl)-L-arginine) and nopaline (N-α-(l, 3-dicarboxypropyl)-L-arginine), is transferred and stably maintained and expressed in the transformed plant cells. This phenomenon can be understood as a ‘genetic colonization’ of the plant cells by bacterial plasmid DNA so that the transformed plant cells will produce and secrete into the medium amino acid derivatives (the opines) that Ti-plasmid carrying agrobacteria can selectively use as carbon and nitrogen sources.
DOI：
--
发表时间：
1977-05
期刊：
Brookhaven symposia in biology
影响因子：
--
作者：
J. Schell;M. V. Montagu
通讯作者：
J. Schell;M. V. Montagu