Immobilisation of DNA to polymerised SU-8 photoresist
Immobilisation of DNA to polymerised SU-8 photoresist
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DOI：
10.1016/j.bios.2005.03.004
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发表时间：
2006-01-15
影响因子：
12.6
通讯作者：
Dufva, M
中科院分区：
文献类型：
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作者：
Marie, R;Schmid, S;Dufva, M
SU-8是一种基于环氧树脂的光敏抗蚀剂，目前用于各种MEMS和芯片实验室应用。在这里，我们展示了一个一步的过程，功能化SU-8与DNA探针。固定程序依赖于DNA与SU-8的直接偶联，并导致表面具有约10 fmol/mm（2）的功能性捕获探针密度，如通过与荧光标记的靶分子的杂交测定所确定的。在商业醛涂覆的玻璃上测量功能性捕获探针的可比密度。DNA探针在杂交前在98 ℃水中孵育10分钟后，杂交性能没有降低，表明DNA和SU-8之间存在共价键。最后，通过直接在SU-8上接触印刷探针溶液，在SU-8上获得高质量的DNA微阵列，展示了在微系统中实现微阵列的简单方法。(c)2005年爱思唯尔B. V.保留所有战斗。
SU-8 is an epoxy-based photosensitive resist, which is currently used for a large variety of MEMS and lab-on-a-chip applications. Here, we demonstrate a one-step process to functionalise SU-8 with DNA probes. The immobilisation procedure relies on direct coupling of DNA to SU-8 and resulted in surfaces with functional capture probe densities of approximately 10 fmol/mm(2) as determined by hybridisation assays with fluorescent labelled target molecules. A comparable density of functional capture probes was measured on commercial aldehyde coated glass. DNA probes did not decrease in hybridisation performance after 10 min incubation in water at 98 degrees C prior to hybridisation, indicating a covalent bond between DNA and SU-8. Finally, DNA microarrays of high quality were obtained on SU-8 by contact printing of probe solution directly on SU-8 demonstrating a simple method for the implementation of microarrays in microsystems. (c) 2005 Elsevier B.V. All fights reserved.