miR-194 Suppresses Proliferation and Migration and Promotes Apoptosis of Osteosarcoma Cells by Targeting CDH2
miR-194 Suppresses Proliferation and Migration and Promotes Apoptosis of Osteosarcoma Cells by Targeting CDH2
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miR-194通过靶向CDH2抑制骨肉瘤细胞的增殖和迁移并促进其凋亡
DOI：
10.1159/000487973
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发表时间：
2018-01-01
影响因子：
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通讯作者：
Chen, Shijie
中科院分区：
文献类型：
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作者：
Miao, Jinglei;Wang, Weiguo;Chen, Shijie
背景/目的：研究表明，MI R-194是一种肿瘤抑制因子，与肿瘤生长和转移有关。本研究旨在揭示miR-194抑制肿瘤的机制。实时定量聚合酶链式反应检测miR-194在骨肉瘤细胞系和骨肉瘤组织中的表达。方法：采用四甲基偶氮唑盐比色法检测细胞增殖能力。用迁移实验和流式细胞仪分别检测细胞的迁移和凋亡情况。用荧光素酶和免疫印迹法检测miR-194对CDH2的调节作用。结果：与正常骨组织相比，骨肉瘤组织中miR-194的表达显著降低。MiR-194过表达可显著抑制骨肉瘤细胞的增殖和迁移，并诱导其凋亡。此外，我们还证明了MI R-194通过下调CDH2的表达来抑制骨肉瘤的恶性行为。结论：这些发现提示miR-194在骨肉瘤中可能是一种肿瘤抑制因子。MIR-194/CDH2可能成为治疗骨肉瘤的新靶点。(C)2018年作者(S)由S.Karger AG，巴塞尔出版
Background/Aims: Studies have shown that mi R-194 functions as a tumour suppressor and is associated with tumour growth and metastasis. This study intends to uncover the mechanism of tumour suppression by miR-194. The expression of miR-194 in osteosarcoma cell lines and tissues were monitored by real-time PCR. Methods: The proliferation ability was examined by MTT assay. Migration and apoptosis of cells were monitored by migration assay and flow cytometry, respectively. The regulation of miR-194 on CDH2 was determined by luciferase assays and western blot assays. Results: The results showed that miR-194 was significantly reduced in osteosarcoma compared with that in normal bone tissue. Overexpression of miR-194 significantly attenuated the proliferation and migration and induced the apoptosis of osteosarcoma cells. Furthermore, we demonstrated that mi R-194 has inhibited the malignant behaviour of osteosarcoma by downregulating CDH2 expression. Conclusions: These findings suggested that miR-194 may act as a tumour suppressor in osteosarcoma. miR-194/CDH2 may be a novel therapeutic target in the treatment of osteosarcoma. (C) 2018 The Author(s) Published by S. Karger AG, Basel