ATR-FTIR spectroscopic investigation of E-coli transconjugants beta-lactams-resistance phenotype
ATR-FTIR spectroscopic investigation of E-coli transconjugants beta-lactams-resistance phenotype
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DOI：
10.1016/s0014-5793(97)00725-4
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发表时间：
1997-07-21
期刊：
影响因子：
3.5
通讯作者：
Manfait, M
中科院分区：
文献类型：
--
作者：
Bouhedja, W;Sockalingum, GD;Manfait, M
采用衰减全反射傅里叶变换红外光谱和聚类分析联用技术对敏感大肠杆菌K12菌株和接合子TEM-1、TEM-2、TEM-3、SHV-2、SHV-3、SHV-4进行了表征，获得了敏感菌株与接合子的良好区分，尽管在SHV和TEM表型的区分方面总体上取得了有限的成功，TEM-2和SHV-3的结果令人信服。观察到的光谱差异归因于共轭过程的全局效应，特别是它们在核酸和碳水化合物吸收区的影响，而不是β-内酰胺酶点突变。(C)1997年欧洲生物化学学会联合会。
Hyphenation of attenuated total reflection Fourier transform infrared spectroscopy and cluster analysis has been used to characterise a susceptible Escherichia coli K12 strain and the transconjugants TEM-1, TEM-2, TEM-3, SHV-2, SHV-3, SHV-4, A good discrimination of the susceptible strain from the transconjugants was obtained, Although a limited success was achieved in the differentiation of SHV and TEM phenotypes in general, results obtained with TEM-2 and SHV-3 were convincing. Spectral differences observed are ascribed to the global effects of the conjugation process, particularly their repercussions in the nucleic acids and carbohydrate absorbing regions, rather than to beta-lactamase point-mutations. (C) 1997 Federation of European Biochemical Societies.