Highly efficient incorporation of amino-reactive dyes into silica particles by a multi-step approach
Highly efficient incorporation of amino-reactive dyes into silica particles by a multi-step approach
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通过多步方法将氨基反应性染料高效地掺入二氧化硅颗粒中
DOI：
10.1016/j.colsurfa.2013.02.064
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发表时间：
2013-06-05
影响因子：
5.2
通讯作者：
Yang, Wensheng
中科院分区：
文献类型：
--
作者：
Liang, Jinglun;Xue, Zheng;Yang, Wensheng
同时水解和缩合的染料-APS（3-氨丙基三乙氧基硅烷）前体和四乙氧基硅烷（TEOS）已被开发作为一种常规的方法，将氨基反应性染料到二氧化硅颗粒。在这种方法中，染料的掺杂效率通常较低（约10%）。10%），这是由于前体和TEOS的竞争性水解和缩合。在这项工作中，我们开发了一种多步骤的方法，有效地将氨基反应性染料掺入二氧化硅颗粒。首先将APS修饰在SiO2粒子表面，然后将染料与粒子表面的氨基共价偶联。进一步生长二氧化硅保护层后，染料掺杂二氧化硅颗粒表现出良好的胶体稳定性、明亮的发光和优异的抗泄漏能力。典型的氨基活性染料如异硫氰酸荧光素（FITC）的掺杂效率高达91%，归因于APS和TEOS的完全分离的水解和缩合过程。这种方法也适用于通过将两种或更多种氨基反应性染料同时偶联到APS改性的二氧化硅颗粒的表面上来制备掺杂有氨基反应性染料的二氧化硅颗粒。(C)2013爱思唯尔有限公司版权所有。
Simultaneous hydrolysis and condensation of the dye-APS (3-aminopropyltriethoxysilane) precursor and tetraethoxysilane (TEOS) has been developed as a conventional method to incorporate the amino-reactive dyes into silica particles. In this method, doping efficiency of the dyes is usually low (ca. 10%) due to the competitive hydrolysis and condensation of the precursor and TEOS. In this work, we developed a multi-step approach for efficient incorporation of the amino-reactive dyes into silica particles. First, APS was modified on surface of the pre-prepared silica particles and then the dyes were covalently coupled with the amino groups of APS on the particle surface. After further growth of silica protection layer, the dye-doped silica particles showed good colloidal stability, bright luminescence and excellent anti-leaking ability. Doping efficiency of the typical amino-reactive dye such as fluorescein isothiocyanate (FITC) was as high as 91% attributed to the complete separated hydrolysis and condensation processes of APS and TEOS. Such an approach is also applicable to the preparation of the multicolor dye-doped silica particles by the simultaneous coupling of two or more amino-reactive dyes onto surface of the APS modified silica particles. (C) 2013 Elsevier B.V. All rights reserved.