LOCALIZATION OF LUTEINIZING-HORMONE RECEPTOR MESSENGER-RIBONUCLEIC-ACID EXPRESSION IN OVARIAN CELL-TYPES DURING FOLLICLE DEVELOPMENT AND OVULATION
LOCALIZATION OF LUTEINIZING-HORMONE RECEPTOR MESSENGER-RIBONUCLEIC-ACID EXPRESSION IN OVARIAN CELL-TYPES DURING FOLLICLE DEVELOPMENT AND OVULATION
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DOI：
10.1210/endo-129-6-3200
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发表时间：
1991-12-01
期刊：
影响因子：
4.8
通讯作者：
NY, T
中科院分区：
文献类型：
--
作者：
PENG, XR;HSUEH, AJW;NY, T
LH 的作用是通过特定的质膜受体介导的，这些受体在生殖周期期间在卵巢中上调和下调。 以PMSG和hCG处理的未成熟大鼠为模型系统，通过Northern印迹和原位杂交研究了卵泡发育、排卵和黄素化过程中不同细胞类型中LH受体mRNA的调控和分布。 在未经治疗的大鼠中，颗粒细胞、卵丘细胞和卵母细胞中的 LH 受体 mRNA 低于检测水平，而鞘细胞中的 LH 受体 mRNA 水平较低。 用 PMSG 刺激后，膜间质细胞中 LH 受体 mRNA 的表达增强，而大三级卵泡的颗粒细胞中受体 mRNA 丰度则出现更显着的增加。 在这些卵泡中，不同颗粒细胞亚群中 LH 受体 mRNA 的丰度存在差异，靠近基底膜的壁颗粒细胞比靠近胃窦的颗粒细胞表现出更高的水平，而卵丘细胞和卵母细胞缺乏可检测的杂交信号。 LH 受体 mRNA 表达的不均匀赋予不同卵巢细胞不同的激素反应性。 在排卵剂量的 hCG 后，LH 受体 mRNA 水平显着下降，特别是在排卵前卵泡的颗粒细胞中，在排卵前达到最低水平。 在排卵卵泡转变为黄体的过程中，LH受体信息的表达再次增加。 我们的结果表明，先前记录的卵巢发育过程中 LH 受体结合活性的调节与 LH 受体转录本的表达相关，这表明 LH 受体基因在排卵期期间以复杂的方式进行调节，以实现细胞特异性表达以及促性腺激素诱导和受体基因活性抑制。
The action of LH is mediated through specific plasma membrane receptors that are both up- and down-regulated in the ovary during the reproductive cycle. Using immature rats treated with PMSG and hCG as a model system, we have studied the regulation and distribution of LH receptor mRNA in different cell types during follicle development, ovulation, and luteinization by Northern blot and in situ hybridization. In untreated rats, LH receptor mRNA was below the detection level in granulosa cells, cumulus cells, and oocytes, while low levels of LH receptor mRNA were found in the thecal cells. After stimulation with PMSG, expression of LH receptor mRNA was enhanced in the thecal-interstitial cells, while a more dramatic increase in receptor mRNA abundance took place in granulosa cells of large tertiary follicles. In these follicles, the abundance of LH receptor mRNA varied among different sub-populations of granulosa cells, with mural granulosa cells close to the basement membrane exhibiting higher levels than granulosa cells located closer to the antrum, and cumulus cells and the oocyte lacking detectable hybridization signal. The uneven expression of LH receptor mRNA endows differnt ovarian cells with varying hormonal responsiveness. After an ovulatory dose of hCG, LH receptor mRNA levels were dramatically decreased, particularly in the granulosa cells of preovulatory follicles, to reach the lowest levels just before ovulation. During the transformation of ovulated follicles into corpora lutea, the expression of LH receptor message was again increased. Our results reveal that the previously documented regulation of the LH receptor-binding activity during ovarian development correlates with expression of the LH receptor transcripts, suggesting that the LH receptor gene is regulated in a complex manner during the periovulatory period to achieve cell-specific expression together with gonadotropin induction and suppression of receptor gene activity.