In vitro and in vivo antioxidant activity of a fructan from the roots of Arctium lappa L.
In vitro and in vivo antioxidant activity of a fructan from the roots of Arctium lappa L.
复制标题
牛蒡根果聚糖的体外和体内抗氧化活性。
DOI：
10.1016/j.ijbiomac.2014.01.062
复制
发表时间：
2014-04-01
影响因子：
8.2
通讯作者：
Gao, Xiangdong
中科院分区：
文献类型：
--
作者：
Liu, Wei;Wang, Jiajia;Gao, Xiangdong
为了从食物中探索新的抗氧化资源，采用热水提取、乙醇沉淀、离子交换层析和凝胶过滤的方法，从牛蒡根中提取水溶性多糖（ALP1）。然后在体外和体内评价ALP1的抗氧化活性。ALP1是一种果糖和葡萄糖的比例为13.0:1.0的果聚糖，平均分子量为4600 Da。ALP1中的键是- >1)-Fruf-(2 ->)、Fruf-(2 ->)和Glcp-(1 ->)。体外抗氧化实验表明，ALP1具有中等的ABTS（中心点+）清除活性，较强的羟基自由基清除活性和较强的铁离子螯合活性。在体内抗氧化实验中，ALP1显著提高了衰老小鼠血清和肝脏中抗氧化酶活性和总抗氧化能力，降低了丙二醛（MDA）水平。这些结果表明，ALP1具有作为一种新型天然抗氧化剂应用于食品工业和制药领域的潜力。(C) 2014 Elsevier B.V.版权所有
To explore new antioxidant resource from food, a water-soluble polysaccharide (ALP1) was extracted and purified from the roots of Arctium lappa L. (A. lappa L.) through hot water extraction followed by ethanol precipitation, ion-exchange chromatography and gel filtration. The antioxidant activity of ALP1 was then evaluated in vitro and in vivo. ALP1 was characterized as a fructan composed of fructose and glucose in the ratio of 13.0:1.0, with an average molecular weight of 4600 Da. The linkages in ALP1 were -> 1)-Fruf-(2 ->, Fruf-(2 -> and Glcp-(1 ->. In vitro antioxidant assays demonstrated that ALP1 possessed moderate ABTS(center dot+) scavenging activity, strong hydroxyl radical scavenging activity and strong ferrous ion chelating activity. In in vivo antioxidant assays, ALP1 administration significantly enhanced antioxidant enzyme activities and total antioxidant capacity, as well as decreased the levels of malondialdehyde (MDA) in both the serum and liver of aging mice. These results suggest that ALP1 has potential as a novel natural antioxidant in food industry and pharmaceuticals. (C) 2014 Elsevier B.V. All rights reserved.