Influenza hemagglutinin mediated fusion of membranes containing poly(ethylene-glycol) grafted lipids: new insights into the fusion mechanism
Influenza hemagglutinin mediated fusion of membranes containing poly(ethylene-glycol) grafted lipids: new insights into the fusion mechanism
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DOI：
10.1016/s0014-5793(99)00333-6
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发表时间：
1999-04-01
期刊：
影响因子：
3.5
通讯作者：
Stegmann, T
中科院分区：
文献类型：
--
作者：
Chams, V;Bonnafous, P;Stegmann, T
使用含有聚乙二醇接枝磷脂酰乙醇胺 (PEG-2000-PE) 的膜研究了覆盖膜的亲水层对流感血凝素 (HA) 介导的融合的影响。这些脂质对 HA 膜相互作用的空间抑制影响了病毒融合（对于具有 114 个乙二醇残基的脂质，半最大抑制为 0.8 mol%；对于 45 个残基 (PEG-2000-PE)，半最大抑制为 3.2 mol%，在该浓度下 PEG 部分采用随机卷曲结构）。含有 3 mol% PEG-2000-PE 的重组病毒膜保留了 40% 的融合活性。因此，膜完全被几纳米的亲水层覆盖，有效的融合是可能的，并且含有PEG-PE的融合病毒体是可行的。 (C) 1999 年欧洲生化学会联合会。
The influence of a hydrophilic layer covering the membrane on influenza hemagglutinin (HA) mediated fusion was investigated using membranes containing poly(ethylene-glycol) grafted phosphatidylethanolamine (PEG-2000-PE). Steric inhibition of HA-membrane interactions by these lipids affected virus fusion (half-maximal inhibition at 0.8 mol% for lipids with 114 ethylene glycol residues, or at 3.2 mol% for 45 residues (PEG-2000-PE), concentrations at which the PEG moieties adopt a random coil structure). Reconstituted viral membranes containing 3 mol% PEG-2000-PE retained 40% of their fusion activity. Therefore, efficient fusion is possible with membranes completely covered by a hydrophilic layer of several nanometers, and fusogenic virosomes containing PEG-PE are feasible. (C) 1999 Federation of European Biochemical Societies.