Proliferation-Promoting Effect of Platelet-Rich Plasma on Human Adipose-Derived Stem Cells and Human Dermal Fibroblasts
Proliferation-Promoting Effect of Platelet-Rich Plasma on Human Adipose-Derived Stem Cells and Human Dermal Fibroblasts
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DOI：
10.1097/prs.0b013e3181882046
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发表时间：
2008-11-01
影响因子：
3.6
通讯作者：
Kusumoto, Kenji
中科院分区：
文献类型：
--
作者：
Kakudo, Natsuko;Minakata, Tatsuya;Kusumoto, Kenji
背景资料：本研究评估了富血小板血浆活化后血小板释放血小板衍生生长因子（PDGF）-AB和转化生长因子（TGF）-β 1的变化，以及活化的富血小板血浆和贫血小板血浆对人脂肪源性干细胞和人真皮成纤维细胞的增殖潜力。使用双离心法制备富血小板血浆，并在每个制备阶段计数血小板数量。用自体凝血酶和氯化钙激活富血小板和贫血小板血浆，并通过酶联免疫吸附试验测定血小板释放的PDGF-AB和TGF-β 1水平。将细胞在补充有5%全血血浆、非活化富血小板血浆、非活化贫血小板血浆、活化富血小板血浆或活化贫血小板血浆的无血清Dulbecco改良Eagle培养基中培养1、4或7天。同时，将这些细胞在补充有1%、5%、10%或20%活化富血小板血浆的无血清Dulbecco改良Eagle培养基中培养1、4或7天。培养的人脂肪来源的干细胞和人皮肤成纤维细胞进行了测定proliferation.Results：血小板丰富的血浆中含有约7.9倍的血小板作为全血，其激活与大量的PDGF-AB和TGF-β 1的释放。添加活化的富血小板或贫血小板血浆显著促进人脂肪源性干细胞和人真皮成纤维细胞的增殖。在培养液中加入5%活化富血小板血浆可最大程度地促进细胞增殖，而加入20%活化富血小板血浆则不能促进细胞增殖。结论：富血小板血浆可促进人脂肪干细胞和人真皮成纤维细胞的增殖。这些结果支持富血小板血浆用于基于细胞的软组织工程和伤口愈合的临床应用。（Plast。重建122：1352，2008）。
Background: This study evaluated changes in platelet-derived growth factor (PDGF)-AB and transforming growth factor (TGF)-beta 1 release from platelets by platelet-rich plasma activation, and the proliferation potential of activated platelet-rich plasma and platelet-poor plasma on human adipose-derived stem cells and human dermal fibroblasts.Methods: Platelet-rich plasma was prepared using a double-spin method, with the number of platelets counted in each preparation stage. Platelet-rich and platelet-poor plasma were activated with autologous thrombin and calcium chloride, and levels of platelet-released PDGF-AB and TGF-beta 1 were determined by enzyme-linked immunosorbent assay. Cells were cultured for 1, 4, or 7 days in serum-free Dulbecco's Modified Eagle Medium supplemented with 5% whole blood plasma, nonactivated platelet-rich plasma, nonactivated platelet-poor plasma, activated platelet-rich plasma, or activated platelet-poor plasma. In parallel, these cells were cultured for 1, 4, or 7 days in serum-free Dulbecco's Modified Eagle Medium supplemented with 1%, 5%, 10%, or 20% activated platelet-rich plasma. The cultured human adipose-derived stem cells and human dermal fibroblasts were assayed for proliferation.Results: Platelet-rich plasma contained approximately 7.9 times as many platelets as whole blood, and its activation was associated with the release of large amounts of PDGF-AB and TGF-beta 1. Adding activated platelet-rich or platelet-poor plasma significantly promoted the proliferation of human adipose-derived stem cells and human dermal fibroblasts. Adding 5% activated platelet-rich plasma to the medium maximally promoted cell proliferation, but activated platelet-rich plasma at 20% did not promote it.Conclusions: Platelet-rich plasma can enhance the proliferation of human adipose-derived stem cells and human dermal fibroblasts. These results support clinical platelet-rich plasma application for cell-based, soft-tissue engineering and wound healing. (Plast. Reconstr. Surg. 122: 1352, 2008.)