Quantitative analysis of the developing rat kidney: Absolute and relative volumes and growth curves
Quantitative analysis of the developing rat kidney: Absolute and relative volumes and growth curves
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发育中的大鼠肾脏的定量分析：绝对和相对体积以及生长曲线
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
I. Gordon
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作者：
J. Bertram;R. Young;Karen Spencer;I. Gordon
永久性肾脏或后肾的发育是一个复杂的过程。在本研究中，在光学显微镜水平上使用体视学方法来估计发育中的大鼠后肾中七个隔室的绝对体积和体积密度，从胚胎第14天（E14）到E21。来自交配时间的 Sprague-Dawley 大鼠的后肾被整个包埋在甲基丙烯酸乙二醇酯中，以 2 μm 彻底切片并用 PAS 染色。在每个年龄对来自三个母亲的三个胚胎的左侧后肾进行分析（总共 72 个后肾）。相对体积乘以总后肾体积以获得绝对体积。在研究的 7 天期间，后肾总体积增加了约 300 倍。在 E14 时，92% 的后肾由未分化的间质组成，而输尿管上皮约占体积的 5%。到 E21，未分化的间质占肾脏的 47%，而输尿管上皮占 9%，发育中的肾单位的各种成分（上皮囊泡、逗号形体、S 形体、肾小球、肾小管）占 43%。生成了描述整个肾脏生长以及七个肾室的绝对和相对生长的预测区间的方程。这些数据为未来研究特定分子在肾脏发育中的作用提供了基线。 Anat Rec 258:128–135, 2000。© 2000 Wiley‐Liss, Inc.
The development of the permanent kidney, or metanephros, is a complex process. In the present study, stereological methods were used at the light microscopic level to estimate the absolute volumes and volume densities of seven compartments in the developing rat metanephros, from embryonic day 14 (E14) to E21. Metanephroi from time‐mated Sprague‐Dawley rats were embedded whole in glycolmethacrylate, exhaustively sectioned at 2 μm and stained with PAS. The left metanephros from three embryos from each of three mothers were analysed at each of the ages (a total of 72 metanephroi). Relative volumes were multiplied by total metanephric volume to obtain absolute volumes. Total metanephric volume increased approximately 300‐fold during the 7‐day period studied. At E14, 92% of the metanephros was composed of undifferentiated mesenchyme, whilst the ureteric epithelium made up approximately 5% of the volume. By E21 the undifferentiated mesenchyme comprised 47% of the kidney, while the ureteric epithelium comprised 9% and the various components of developing nephrons (epithelial vesicles, comma‐shaped bodies, S‐shaped bodies, glomeruli, tubules) comprised 43%. Equations with prediction intervals describing the growth of the whole kidney as well as the absolute and relative growth of the seven kidney compartments were generated. These data provide a baseline for future studies on the roles of specific molecules in renal development. Anat Rec 258:128–135, 2000. © 2000 Wiley‐Liss, Inc.