DNA damage as an indicator of chronic stress: Correlations with corticosterone and uric acid
DNA damage as an indicator of chronic stress: Correlations with corticosterone and uric acid
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DOI：
10.1016/j.cbpa.2018.10.007
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发表时间：
2019-01-01
影响因子：
2.3
通讯作者：
Romero, L. Michael
中科院分区：
文献类型：
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作者：
Gormally, Brenna M. G.;Fuller, Rory;Romero, L. Michael
皮质酮在不同物种和环境中不会以一致的方式变化，这使得它很难作为慢性压力的指标。我们评估了DNA损伤作为一个潜在的指标，可能是一个更综合的压力测量与健康的直接联系。我们捕获了自由生活的家麻雀，立即采集了血液样本，并将它们转移到实验室，使它们暴露在长期的圈养压力下。然后每两周采集一次血液和体重样本，持续4周。我们立即使用彗星试验评估红细胞中的DNA损伤，随后定量皮质酮。在单独的一组鸡中分析尿酸。我们发现，鸟类最初失去了，但在圈养过程中开始恢复体重。DNA损伤在圈养的前10天达到峰值，并且大部分保持升高。然而，损伤的细胞分布随着时间的推移发生了很大变化;大多数细胞早期表现出低水平的损伤，在损伤高峰期表现出高和低DNA损伤的双峰分布，在4周结束时表现出宽单峰分布的损伤。此外，皮质酮增加，并保持在同一时期内升高和尿酸下降，并保持耗尽。虽然分子（DNA损伤）和内分泌（皮质酮）标志物在4周内显示出相似的反应特征，但它们不相关，表明它们反映了潜在生理学的不同方面。这些数据提供了令人信服的初步证据，表明DNA损伤有可能成为慢性压力的另一个指标。
Corticosterone does not change in consistent ways across species and contexts, making it challenging to use as an indicator of chronic stress. We assessed DNA damage as a potential metric that could be a more integrative stress measurement with direct links to health. We captured free-living house sparrows, took an immediate blood sample, and transferred them to the laboratory, exposing them to the chronic stress of captivity. Biweekly blood and weight samples were then taken for 4 weeks. We immediately assessed DNA damage in red blood cells using the comet assay and later quantified corticosterone. Uric acid was analyzed in a separate group of birds. We found that birds initially lost, but began to regain weight over the course of captivity. DNA damage peaked within the first 10 days of captivity, and mostly remained elevated. However, the cellular distribution of damage changed considerably over time; most cells showed low levels of damage early, a bimodal distribution of high and low DNA damage during the peak of damage, and a wide unimodal distribution of damage at the end of the 4 weeks. Furthermore, corticosterone increased and remained elevated and uric acid decreased and remained depleted over the same period. Although both a molecular (DNA damage) and an endocrine (corticosterone) marker showed similar response profiles over the 4 weeks, they were not correlated, suggesting they reflect different aspects of the underlying physiology. These data provide convincing preliminary evidence that DNA damage has potential to be an additional indicator of chronic stress.