Culture Temperature Affects Redifferentiation and Cartilaginous Extracellular Matrix Formation in Dedifferentiated Human Chondrocytes
Culture Temperature Affects Redifferentiation and Cartilaginous Extracellular Matrix Formation in Dedifferentiated Human Chondrocytes
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DOI：
10.1002/jor.22808
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发表时间：
2015-05-01
影响因子：
2.8
通讯作者：
Kuroki, Hiroshi
中科院分区：
文献类型：
--
作者：
Ito, Akira;Aoyama, Tomoki;Kuroki, Hiroshi
迄今为止，关于温度如何影响人类软骨细胞的表型和代谢的研究很少。因此，本研究的目的是阐明培养温度对软骨细胞再分化和细胞外基质（ECM）形成的影响，使用去分化的成熟人软骨细胞在体外。将去分化软骨细胞在团块培养系统中培养长达21天。将沉淀物随机分为三组，具有不同的培养温度（32、37和41 ° C）。通过湿重、信使核糖核酸（mRNA）、组织学和生化分析评价软骨细胞再分化和ECM形成。结果表明，37 ℃下的湿重和II型胶原A1和软骨寡聚基质蛋白的mRNA表达高于32 ℃下的相应值。组织学和生物化学分析表明，与32 ℃相比，37 ℃促进了II型胶原和蛋白多糖的合成，而41 ℃则明显抑制了II型胶原和蛋白多糖的合成。总之，本文获得的结果表明，温度影响软骨细胞再分化和ECM形成，并且温度的调节因此可能代表调节软骨细胞的表型和生物合成活性的有利手段。(c)2015骨科研究学会。出版社：Wiley Periodicals，Inc. J Orthop Res 33：633-639，2015.
To date, there have been few studies on how temperature affects the phenotype and metabolism of human chondrocytes. Thus, the purpose of this study was to elucidate the effects of culture temperature on chondrocyte redifferentiation and extracellular matrix (ECM) formation using dedifferentiated mature human chondrocytes in vitro. Dedifferentiated chondrocytes were cultured in a pellet culture system for up to 21days. The pellets were randomly divided into three groups with different culture temperature (32, 37, and 41 degrees C). Chondrocyte redifferentiation and ECM formation were evaluated by wet weight, messenger ribonucleic acid (mRNA), histological, and biochemical analyses. The results showed that the wet weight and the mRNA expressions of collagen type II A1 and cartilage oligomeric matrix protein at 37 degrees C were higher than the corresponding values at 32 degrees C. The histological and biochemical analyses revealed that the syntheses of type II collagen and proteoglycan were promoted at 37 degrees C compared to those at 32 degrees C, whereas they were considerably inhibited at 41 degrees C. In conclusion, the results obtained herein indicated that temperature affects chondrocyte redifferentiation and ECM formation, and modulation of temperature might thus represent an advantageous means to regulate the phenotype and biosynthetic activity of chondrocytes. (c) 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:633-639, 2015.