Field evaluation of a test for praziquantel resistance in Schistosoma sp.
Field evaluation of a test for praziquantel resistance in Schistosoma sp.
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DOI：
10.1016/s0304-4017(03)00036-0
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发表时间：
2003-04-02
影响因子：
2.6
通讯作者：
Coles, GC
中科院分区：
文献类型：
--
作者：
Kenworthy, JD;Ye, P;Coles, GC
血吸虫吡喹酮耐药简易快速检测法的建立包括暴露于吡喹酮后毛蚴形状的变化。在江西省的一个野外实验室和上海的一个研究实验室对自然感染毛蚴的山羊卵进行了孵化试验。两个实验室的平均值无显著差异，但两个实验室个体样本之间的差异表明，常规耐药诊断需要划定剂量。确认日本血吸虫已检测到耐药性必须等待耐药分离株的分离。试验结果表明，吡喹酮25 mg/kg对山羊感染和水牛化疗均敏感。第一次治疗的治愈率为95%，第二次治疗的治愈率为100%，与之前在人类患者中发现的相似。(C)2003 Elsevier Science B. V.保留所有权利。
A simple rapid test for detecting praziquantel resistance in Schistosoma sp., involving change in shape of miracidia on exposure to praziquantel, was evaluated in China. Tests on miracidia hatched from eggs collected from naturally infected goats were run in a field laboratory in Jiangxi Province and a research laboratory in Shanghai. The mean values in the two laboratories were not significantly different, but the variation between individual samples in the two laboratories suggests that a delineating dose will be required for routine diagnosis of resistance. Confirmation that resistance would have been detected in Schistosoma japonicum must await the isolation of a resistant isolate. The tests suggested that the infection in the goats was susceptible to praziquantel as did chemotherapy of water buffaloes with 25 mg/kg. This gave a 95% cure rate on the first treatment and 100% with a second treatment, similar to that found previously in human patients. (C) 2003 Elsevier Science B.V. All rights reserved.