CD36 does not play a direct role in HDL or LDL metabolism.
CD36 does not play a direct role in HDL or LDL metabolism.
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DOI：
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发表时间：
2001-08
影响因子：
6.5
通讯作者：
W. D. Villiers;Lei Cai;N. Webb;M. Beer;D. Westhuyzen;F. D. Beer
中科院分区：
文献类型：
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作者：
W. D. Villiers;Lei Cai;N. Webb;M. Beer;D. Westhuyzen;F. D. Beer
CD 36和I型B类清道夫受体（SR-BI）都是B类清道夫受体，其识别多种配体，包括氧化低密度脂蛋白（oxLDL）、HDL、阴离子磷脂和凋亡细胞。在这项研究中，我们研究了小鼠CD 36（mCD 36）作为生理性脂蛋白受体的作用。我们比较了各种脂蛋白颗粒与通过腺病毒介导的基因转移在COS细胞中表达的mCD 36和mSR-BI的关联。mCD 36以高亲和力结合人oxLDL和小鼠HDL。人LDL与mCD 36的结合较差，表明mCD 36不太可能在LDL代谢中发挥重要作用。评估了mCD 36介导从受体结合HDL选择性摄取胆固醇酯（CE）的能力。与mSR-BI相比，mCD 36无效地介导CE的选择性摄取。通过腺病毒介导的基因转移在C57 BL/6小鼠中肝脏过表达mCD 36并没有导致血浆LDL和HDL水平的显著改变。我们的结论是，mCD 36，而能够结合高密度脂蛋白与高亲和力，并没有显着的HDL或LDL代谢。
CD36 and scavenger receptor class B, type I (SR-BI) are both class B scavenger receptors that recognize a broad variety of ligands, including oxidized low density lipoprotein (oxLDL), HDL, anionic phospholipids, and apoptotic cells. In this study we investigated the role of mouse CD36 (mCD36) as a physiological lipoprotein receptor. We compared the association of various lipoprotein particles with mCD36 and mSR-BI expressed in COS cells by adenovirus-mediated gene transfer. mCD36 bound human oxLDL and mouse HDL with high affinity. Human LDL bound poorly to mCD36, indicating that mCD36 is unlikely to play a significant role in LDL metabolism. The ability of mCD36 to mediate the selective uptake of cholesteryl esters (CE) from receptor-bound HDL was assessed. In comparison with mSR-BI, mCD36 inefficiently mediated the selective uptake of CE. Hepatic overexpression of mCD36 in C57BL/6 mice by adenovirus-mediated gene transfer did not result in significant alterations in plasma LDL and HDL levels. We conclude that mCD36, while able to bind HDL with high affinity, does not contribute significantly to HDL or LDL metabolism.