Preparation and properties of nickel hemoglobin.
Preparation and properties of nickel hemoglobin.
复制标题
镍血红蛋白的制备及性质。
作者：
K. Alston;A. Schechter;J. Arcoleo;J. Greer;G. Parr;F. Friedman
血红蛋白A与镍原卟啉IX（NiHbA）重建已制备和表征。其反应动力学与p-汞苯甲酸和与触珠蛋白，吸收光谱和圆二色谱，和X-射线晶体学性质已被调查作为探针的结构构象。结果表明，NiHbA以类似于HbA的脱氧或T状态的结构存在。NiHbA及其衍生物可作为今后研究血红蛋白变构变化的有用模型。
Hemoglobin A reconstituted with nickel protoporphyrin IX (NiHbA) has been prepared and characterized. Kinetics of its reaction with p-mercuribenzoate and with haptoglobin, absorption and circular dichroism spectra, and x-ray crystallographic properties have been investigated as probes of its structural conformation. The results suggest that NiHbA exists in a structure that is similar to the deoxy, or T-state of HbA. It is proposed that NiHbA and its derivatives may serve as a useful model for future studies of hemoglobin allosteric changes.
通过选择性蛋白水解探测触珠蛋白上的血红蛋白结合位点。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Lustbader,JW;Arcoleo,JP;Birken,S;Greer,J
通讯作者：
Greer,J