Molecular Epidemiology of Geographically Dispersed Vibrio cholerae, Kenya, January 2009-May 2010
Molecular Epidemiology of Geographically Dispersed Vibrio cholerae, Kenya, January 2009-May 2010
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DOI：
10.3201/eid1806.111774
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发表时间：
2012-06-01
影响因子：
11.8
通讯作者：
Stine, O. Colin
中科院分区：
文献类型：
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作者：
Mohamed, Ahmed Abade;Oundo, Joseph;Stine, O. Colin
自 1971 年以来，肯尼亚爆发了多起霍乱疫情。为了更全面地了解肯尼亚霍乱的流行病学，我们分析了包含可变串联重复序列的 5 个基因座的 170 个霍乱弧菌 O1 分离株之间的遗传关系。这些分离株是在 2009 年 1 月至 2010 年 5 月期间从全国各个地理区域收集的。这些分离株在遗传上分为 5 个克隆复合体，每个克隆复合体包含一系列基因型，这些基因型的不同之处在于单个基因座的等位基因变化。没有观察到分离株的地理位置与其基因型之间存在明显的相关性。然而，克隆复合体发生了地理分化。我们的分析表明，霍乱弧菌的多个遗传谱系同时感染肯尼亚人。这一发现与地方性环境储存库中同时出现的霍乱弧菌多个不同遗传谱系一致，而不是最近由旅行者引入和传播。
Numerous outbreaks of cholera have occurred in Kenya since 1971. To more fully understand the epidemiology of cholera in Kenya, we analyzed the genetic relationships among 170 Vibrio cholerae O1 isolates at 5 loci containing variable tandem repeats. The isolates were collected during January 2009-May 2010 from various geographic areas throughout the country. The isolates grouped genetically into 5 clonal complexes, each comprising a series of genotypes that differed by an allelic change at a single locus. No obvious correlation between the geographic locations of the isolates and their genotypes was observed. Nevertheless, geographic differentiation of the clonal complexes occurred. Our analyses showed that multiple genetic lineages of V. cholerae were simultaneously infecting persons in Kenya. This finding is consistent with the simultaneous emergence of multiple distinct genetic lineages of V. cholerae from endemic environmental reservoirs rather than recent introduction and spread by travelers.