Spatiotemporal localization of germ plasm RNAs during zebrafish oogenesis
Spatiotemporal localization of germ plasm RNAs during zebrafish oogenesis
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DOI：
10.1016/j.mod.2007.01.003
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发表时间：
2007-04-01
影响因子：
2.6
通讯作者：
Inoue, Kunio
中科院分区：
文献类型：
--
作者：
Kosaka, Kyoko;Kawakami, Koichi;Inoue, Kunio
在斑马鱼中，原始生殖细胞（PGCs）是由一个专门的母体细胞质，胚质，它形成在4细胞胚胎的卵裂沟的远端。种质包括来自生殖系特异性基因如vasa和nanos 1的母体mRNA，并且植物定位的dazl RNA也被并入种质中。然而，对种质组分的分布和组装机制，特别是在卵子发生过程中的分布和组装机制知之甚少。在这里，我们报告的种质RNA vasa，nanos1，和dazl共定位与线粒体云（MC），并在早期卵子发生过程中被运送到植物皮质。在斑马鱼卵母细胞中，我们发现在非洲爪蟾Xcat 2基因的3'非翻译区（3'UTR）中发现了一个线粒体云定位元件（MCLE），该元件可以引导RNA通过MC定位到植物皮层。此外，RNA结合蛋白爱马仕是斑马鱼卵母细胞MC的一个组成部分，就像非洲爪蟾一样。此外，我们提供的证据表明，dazl 3 'UTR具有至少三种类型的顺式作用元件，这些元件指导定位过程中的多个步骤：MC定位、植物皮质的锚定和卵裂沟的定位。两者合计，数据表明，MC功能作为一个保守的功能，参与运输的种质RNA在非洲爪蟾和斑马鱼卵母细胞。此外，我们建议，种质组件组装在一个逐步和时空调节的方式在斑马鱼卵子发生和早期胚胎发生。(c)2007爱思唯尔爱尔兰有限公司保留所有权利。
In zebrafish, primordial germ cells (PGCs) are determined by a specialized maternal cytoplasm, the germ plasm, which forms at the distal ends of the cleavage furrows in 4-cell embryos. The germ plasm includes maternal mRNAs from the germline-specific genes such as vasa and nanos1, and vegetally localized dazl RNA is also incorporated into the germ plasm. However, little is known about the distributions and assembly mechanisms of germ plasm components, especially during oogenesis. Here we report that the germ plasm RNAs vasa, nanos1, and dazl co-localize with the mitochondrial cloud (MC) and are transported to the vegetal cortex during early oogenesis. We found that a mitochondrial cloud localization element (MCLE) previously identified in the 3' untranslated region (3'UTR) of Xenopus Xcat2 gene can direct RNA localization to the vegetal cortex via the MC in zebrafish oocytes. In addition, the RNA-binding protein Hermes is a component of the MC in zebrafish oocytes, as is the case in Xenopus. Moreover, we provide evidence that the dazl 3'UTR possesses at least three types of cis-acting elements that direct multiple steps in the localization process: MC localization, anchorage at the vegetal cortex, and localization at the cleavage furrows. Taken together, the data show that the MC functions as a conserved feature that participates in transport of the germ plasm RNAs in Xenopus and zebrafish oocytes. Furthermore, we propose that the germ plasm components are assembled in a stepwise and spatiotemporally-regulated manner during oogenesis and early embryogenesis in zebrafish. (c) 2007 Elsevier Ireland Ltd. All rights reserved.