SPECIFIC MITOCHONDRIAL PROTEINS IN POLLEN - PRESENCE OF AN ADDITIONAL ATP SYNTHASE BETA-SUBUNIT
SPECIFIC MITOCHONDRIAL PROTEINS IN POLLEN - PRESENCE OF AN ADDITIONAL ATP SYNTHASE BETA-SUBUNIT
复制标题
DOI：
10.1073/pnas.90.13.5934
复制
发表时间：
1993-07-01
影响因子：
11.1
通讯作者：
VEDEL, F
中科院分区：
文献类型：
--
作者：
DEPAEPE, R;FORCHIONI, A;VEDEL, F
设计了一种从异源四倍体烟草的母本--二倍体烟草中提取花粉线粒体的方法。大多数细胞器形态完整，能够参与细胞器线粒体(MT)的蛋白质合成。双向蛋白质电泳法显示，叶片和花粉的mt蛋白之间存在着大量的数量差异。此外，在花粉中还发现了由核基因或线粒体基因编码的额外的mt多肽，命名为R(生殖)。最丰富的R多肽R1(M(R)53,000，pI 5.6)是核编码的，与膜结合，并与针对线粒体ATP合成酶(ATPase)的β亚基的抗体发生交叉反应。N端微序列分析表明，叶片中存在的两个ATPaseβ亚基(Beta1和Beta2)和R1花粉特异亚基是由不同的基因编码的。在矮牵牛花粉线粒体中也观察到类似的附加ATPaseβ亚基，这表明这种多肽对茄科植物雄配子体的发育具有普遍的重要性。
A protocol was designed to obtain a pure fraction of pollen mitochondria from the diploid species Nicotiana sylvestris, the female parent of the allotetraploid Nicotiana tabacum. Most organelles were morphologically intact and able to perform in organello mitochondrial (mt) protein synthesis. As revealed by two-dimensional protein electrophoresis, numerous quantitative differences exist between leaf and pollen mt proteins. Moreover, additional mt polypeptides, named R (for reproductive), encoded by either nuclear or mitochondrial genes, are found in pollen. The most abundant R polypeptide, R1 (M(r) 53,000, pI 5.6), is nuclearly encoded, is membrane bound, and cross-reacts with an antibody directed against the beta subunit of the mt ATP synthase (ATPase). N-terminal microsequence analysis showed that the two ATPase beta subunits present in leaves (beta1 and beta2) and the R1 pollen-specific subunit are encoded by distinct genes. A similar additional ATPase beta subunit was observed in pollen mitochondria from Petunia, suggesting that this polypeptide is of general importance for male gametophytic development in Solanaceaes.