Royal jelly and its unique fatty acid, 10-hydroxy-trans-2-decenoic acid, promote neurogenesis by neural stem/progenitor cells in vitro
Royal jelly and its unique fatty acid, 10-hydroxy-trans-2-decenoic acid, promote neurogenesis by neural stem/progenitor cells in vitro
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DOI：
10.2220/biomedres.28.261
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发表时间：
2007-10-01
影响因子：
1.2
通讯作者：
Furukawa, Shoei
中科院分区：
文献类型：
--
作者：
Hattori, Noriko;Nomoto, Hiroshi;Furukawa, Shoei
神经干/祖细胞（NSC）在含有碱性成纤维细胞生长因子（FGF-2）的培养基中作为神经球而活跃地增殖，但在FGF-2-FGF培养基中开始分化为神经元、星形胶质细胞或少突胶质细胞。皇家浆（RJ）提取物不仅显著增加了对III类β-微管蛋白（Tuj 1）免疫反应的神经元在总细胞群中的百分比，而且显著增加了对神经胶质酸性蛋白（GFAP）免疫反应的星形胶质细胞和对由NSC产生的2 '，3'-环核苷酸3-磷酸二酯酶（CNE 3）免疫反应的少突胶质细胞在总细胞群中的百分比，但降低了巢蛋白阳性NSC的百分比。这些结果突出了RJ的新颖且突出的性质，即，它促进所有类型的脑细胞（神经元，星形胶质细胞和少突胶质细胞）的分化。另一方面，10-羟基-反式-2-癸烯酸（HDEA），RJ的不饱和脂肪酸的特性，增加神经元的产生，减少从神经干细胞的星形胶质细胞。这些观察结果表明，RJ含有多种成分，不同地影响神经元和/或神经胶质细胞谱系和HDEA是这样的RJ，促进神经干细胞的神经发生的组件之一。
Neural stem/progenitor cells (NSCs) proliferate vigorously as neurospheres in medium containing basic fibroblast growth factor (FGF-2), but start differentiating into neurons, astrocytes or oligodendrocytes in FGF-2-ftee medium. An extract of royal jelly (RJ) significantly increased the percentage in the total cell population of not only neurons immunoreactive for class III beta-tubulin (Tuj1) but also astrocytes immunoreactive for glial fibrillary acidic protein (GFAP), and oligodendrocytes immunoreactive for 2','3'-cyclic nucleotide 3-phosphodiesterase (CNPase) generated from NSCs, but decreased that of nestin-positive NSCs. These results highlight a novel and outstanding property of the RJ, i.e., that it facilitates the differentiation of all types of brain cells (neurons, astrocytes, and oligodendrocytes). On the other hand, 10-hydroxy-trans-2-decenoic acid (HDEA), an unsaturated fatty acid characteristic of RJ, increased the generation of neurons and decreased that of astrocytes from NSCs. These observations suggest that RJ contains plural components that differently influence neuronal and/or glial lineages and that HDEA is one of such components of RJ that facilitates neurogenesis by NSCs.