Preparation and characterization of molecularly imprinted monolithic column based on 4-hydroxybenzoic acid for the molecular recognition in capillary electrochromatography
Preparation and characterization of molecularly imprinted monolithic column based on 4-hydroxybenzoic acid for the molecular recognition in capillary electrochromatography
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DOI：
10.1016/j.aca.2004.07.001
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发表时间：
2004-10
影响因子：
6.2
通讯作者：
Zhaosheng Liu;Yan-Li Xu;Chao Yan;R. Gao
中科院分区：
文献类型：
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作者：
Zhaosheng Liu;Yan-Li Xu;Chao Yan;R. Gao
以4-羟基苯甲酸（4-HBA）为模板分子，建立了一种制备分子印迹整体聚合物（MIPs）的新方法。采用高浓度单体预聚合的策略，以满足极性印迹分子的溶解性和减少复合物形成过程中的干扰。以甲基丙烯酸（MAA）和乙二醇二甲基丙烯酸酯（乙二醇二甲基丙烯酸酯）为原料，甲苯-异辛烷混合溶剂为致孔剂，在极性模板分子4-HBA存在下，通过原位热引发共聚反应制备了印迹聚合物毛细管整体柱。在此整体柱上，考察了CEC参数对电渗流和4-HBA保留的影响。印迹分子4-HBA的柱效为13,000板/m。HBA异构体的分离度为5.0，对4-HBA具有良好的分子识别。
A novel prepared method of molecularly imprinted monolithic polymers (MIPs) using 4-hydroxybenzoic acid (4-HBA) as templates for capillary electrochromatography (CEC) was developed. A strategy of high concentration of monomers in the pre-polymerization mixture was used to fulfil the solubility of polar imprinted molecule and reduction of the interference during complex formation. The imprinted polymer capillary monolithic column was synthesized by an in situ therm-initiated copolymerization of methacrylic acid (MAA) and ethylene glycol dimethacrylate with a mixture of toluene-isooctane as a porogenic solvent in the presence of a polar model imprinting molecule, 4-HBA. On the resultant MIP monolithic column, the effect of parameter of CEC on electroosmotic flow (EOF) and the retention of 4-HBA was investigated. The column efficiency of the imprinted molecule, 4-HBA, was 13,000plates/m. The resolution of isomers of HBA was 5.0 and good molecular recognition was achieved for 4-HBA.