DNA-binding protein associated with herpes simplex virus DNA polymerase
DNA-binding protein associated with herpes simplex virus DNA polymerase
复制标题
与单纯疱疹病毒 DNA 聚合酶相关的 DNA 结合蛋白
作者：
P. Vaughan;D. Purifoy;K. Powell
由许多不同实验室制备的单纯疱疹病毒2型DNA聚合酶的纯化制剂总是含有至少两种多肽。主要的一种，分子量约为150，000，与聚合酶活性有关。第二种蛋白质，分子量约为54，000，我们以前将其命名为ICSP 34，35，现已纯化。纯化的蛋白质已用于制备抗血清（多克隆兔血清和单克隆抗体）。这些试剂已被用来表征蛋白质，以证明其完全不同的地图位置从单纯疱疹病毒基因组上的DNA聚合酶，并证明两种多肽之间的密切关联。
Purified preparations of herpes simplex virus type 2 DNA polymerase made by many different laboratories always contain at least two polypeptides. The major one, of about 150,000 molecular weight, has been associated with the polymerase activity. The second protein, of about 54,000 molecular weight, which we previously designated ICSP 34, 35, has now been purified. The purified protein has been used to prepare antisera (both polyclonal rabbit serum and monoclonal antibodies). These reagents have been used to characterize the protein, to demonstrate its quite distinct map location from that of the DNA polymerase on the herpes simplex virus genome, and to demonstrate the close association between the two polypeptides.