Apoptosis in the epidermis.

Apoptosis in the epidermis.
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表皮细胞凋亡。

DOI:
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发表时间:
2005
影响因子:
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通讯作者:
H. Mizutani
H. Mizutani
中科院分区:
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文献类型:
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作者:
K. Yamanishi;Chun;H. Mizutani

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角质化是细胞凋亡的一种特殊形式,其产生角质层伴随角质细胞死亡。角质形成细胞的凋亡不仅发生在正常的角质形成过程中,而且还响应于各种细胞内或细胞外的死亡刺激,如遗传缺陷或UVB。通常,细胞凋亡的特征在于染色质浓缩、膜起泡、细胞皱缩、凋亡小体的形成和脱氧核糖核酸片段化。缺口末端标记技术是一种检测DNA片段化的方法,可用于评估组织或细胞中凋亡细胞的存在。在这一章中,一个实用的程序缺口结束标记石蜡包埋切片的皮肤标本进行了说明。
Keratinization is a specialized form of apoptosis that produces the stratum corneum concomitant with keratinocyte cell death. Apoptosis of keratinocytes occurs not only during normal keratinization but also in response to various intracellular or extracellular death stimuli, such as genetic defects or UVB. In general, apoptosis is characterized by chromatin condensation, membrane blebbing, cell shrinkage, formation of apoptotic bodies, and deoxyribonucleic acid fragmentation. The nick-end labeling technique is a method to detect DNA fragmentation and is useful to assess the presence of apoptotic cells in tissues or cells. In this chapter, a practical procedure for nick-end labeling using paraffin-embedded sections of skin specimens is described.
DOI: 10.1111/1523-1747.ep12478519
发表时间: 1991-07-01
影响因子: 6.5
作者:
MCCALL, CA;COHEN, JJ
通讯作者: COHEN, JJ