Modulation of the liver specific phenotype in the human hepatoblastoma line Hep G2.
Modulation of the liver specific phenotype in the human hepatoblastoma line Hep G2.
复制标题
人肝母细胞瘤系 Hep G2 中肝脏特异性表型的调节。
DOI：
10.1007/bf02626182
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Darlington,GJ
中科院分区：
文献类型：
--
作者：
Kelly,JH;Darlington,GJ
人肝母细胞瘤细胞系Hep G2在体外调节基因表达，以响应培养密度的增加。可以定义两个发展阶段。在低密度(<；200,000个/cm~2)下，培养24小时，表现出胎肝细胞的几个特征，包括甲胎蛋白合成增加，白蛋白合成减少，胎儿醛缩酶和丙酮酸激酶同工酶占优势，去唾液酸糖蛋白的细胞表面受体水平降低。融合的高密度培养的Hep G2(1×106个/cm2)有193小时的倍增时间，白蛋白产量增加4倍，成体醛缩酶和丙酮酸激酶同工酶水平增加，去唾液酸糖蛋白受体增加。白蛋白和甲胎蛋白合成的改变反映在信使RNA水平和这两个基因的相对转录的变化上。Hep G2为胎儿/成人发育或肝脏再生过程中肝脏表型的调节提供了一种细胞培养模型。
The human hepatoblastoma line Hep G2 modulates gene expression in vitro in response to increasing culture density. Two stages of growth can be defined. At low density (<200,000 cells/cm2) the cultures have a doubling time of 24 h and exhibit several characteristics of fetal liver cells, including increased synthesis of alphafetoprotein, reduced synthesis of albumin, a predominance of the fetal isoenzymes of both aldolase and pyruvate kinase and a reduced level of the cell surface receptor for asialoglycoproteins. Confluent, high density cultures of Hep G2 (>1×106cells/cm2) have a doubling time of 193 h, a four fold higher level of albumin production, increased levels of the adult isoenzymes of aldolase and pyruvate kinase and increased asialoglycoprotein receptor. The alteration in albumin and alphafetoprotein synthesis was reflected by changes in the messenger RNA levels and the relative transcription of these two genes. Hep G2 provides a cell culture model for the modulation of the liver phenotype which occurs during fetal/adult development or during liver regeneration.