Ontogeny of central regulation of visceral reflex activity in the rabbit.
Ontogeny of central regulation of visceral reflex activity in the rabbit.
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兔子内脏反射活动中枢调节的个体发育。
DOI：
10.1152/ajplegacy.1967.212.2.335
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发表时间：
1967
期刊：
影响因子：
--
通讯作者：
W. Bradley
中科院分区：
文献类型：
--
作者：
W. Bradley
方法选用新西兰白色家兔进行研究，精确记录出生后年龄。动物年龄范围为1天至6个月。对动物进行称重，测量头臀长度，引出肌腱和翻正反射，测定四肢被动运动的张力，并观察跳跃或爬行的能力。用开放式乙醚麻醉家兔，并进行气管造口术。聚乙烯管（Clay亚当斯公司，纽约市）通过尿道插入膀胱（22）。后一种方法是切除部分骨化的耻骨联合，部分分离远端尿道，将聚乙烯管插入膀胱后，用远端丝线固定。尿道周围的结扎将结扎线放置在尽可能远的位置，以避免损伤膀胱的血管和神经供应。聚乙烯管的远端连接到T形套管。在套管的一个臂上连接变速恒容排量泵（蠕动泵，型号600-1200，哈佛仪器公司，多佛，马萨诸塞州）。将应变仪（型号P23，斯坦森仪器公司，加利福尼亚州洛杉矶）以及应变仪放大器，其输出记录在变速条形图记录器（Mosely Co.，Pasadena，Calif.在轻度乙醚麻醉下，以俯卧位缓慢、恒定输注温盐水（0.9%NaCl）溶液（在1至8日龄动物中为0.021 -0.042 ml/min），进行对照膀胱测压。在膀胱测压结束时，泵入膀胱的容量与从膀胱排出的容量之间的差异归因于肾滤过。在引起高振幅（10-40 cm水）逼尿肌反射反应后终止充盈程序。在颈部进行椎板切除和脊髓横断-
METHODSNew Zealand white rabbits were selected for study with precise documentation of postnatal age. Animals ranged in age from I day to 6 months. Animals were weighed, crown-rump length measured, tendon and righting reflexes elicited, tone on passive movement of the extremities determined, and the ability to hop or crawl observed. Rabbits were anesthetized with open ether and a tracheostomy was performed. A polyethylene tube (Clay Adams Co., New York City) of appropriate size (PE-go in I-to 5-day-old animals; PE-200 in 6 to IO-day-old animals; and PE-320 in adults) was inserted through the urethra into the bladder (22). The latter was performed after removal of the partially ossified pubic symphysis and partial division of the distal urethra.After insertion of the polyethylene tube into the bladder, the tube was secured by a distal silk l. igature around the urethra. The ligature was placed as distal as possible to avoid injury to the vascular and nerve supply to the bladder. The distal end of the polyethylene tubing was connected to a T-shaped cannula. To one arm of the cannula was connected the output of a variable-speed, constant-volume displacement pump (peristaltic pump, model 600-1200, Harvard Apparatus Co., Dover, Mass.). To the other arm of the T-shaped cannula was connected a strain gauge (model P23, Statham Instruments, Inc., Los Angeles, Calif.) and strain-gauge amplifier whose output was recorded on a variable-speed strip-chart recorder (Mosely Co., Pasadena, Calif.). Control cystometrograms were carried out under light ether anesthesia with slow, constant infusion (0.02 1-0.042 ml/min in the I-to s-day-old animals) of warm saline (0.9% NaCl) solution in the prone position. Differences between volume pumped into the bladder and amount drained from the bladder at the end of a cystometrogram were attributed to renal filtration. Filling procedures were terminated after a high-amplitude(10-40 cm water) detrusor reflex response was elicited. Laminectomy and transection of the spinal cord at the cervico-