VARIATIONS IN HUMAN CORNEAL ENDOTHELIAL-CELL MORPHOLOGY AND PERMEABILITY TO FLUORESCEIN WITH AGE
VARIATIONS IN HUMAN CORNEAL ENDOTHELIAL-CELL MORPHOLOGY AND PERMEABILITY TO FLUORESCEIN WITH AGE
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DOI：
10.1016/0014-4835(88)90021-8
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发表时间：
1988-07-01
影响因子：
3.4
通讯作者：
BRUBAKER, RF
中科院分区：
文献类型：
--
作者：
CARLSON, KH;BOURNE, WM;BRUBAKER, RF
对80例正常人（年龄5-79岁）进行了局部应用荧光素的荧光光度测定和内皮细胞照相。记录内皮细胞形态和功能、房水流量和眼内压的变化。平均内皮细胞大小为332.3 ± 0.5。46.3μ m2。在80年中，测量到内皮细胞大小增加了28%（r = 0.53，P < 0.001）。细胞大小的变异系数随年龄增长而增大（r = 0.41，P < 0.001）。随着年龄的增长，六边形内皮细胞的比例下降14%（r =-0.48，P < 0.001），五边形和七边形内皮细胞的比例分别增加50%（r = 0.44，P < 0.001）和40%（r = 0.33，P < 0.002）。内皮细胞对荧光素的平均渗透性为4.03 ± 0.05。0.63. times. 10-4 cm min-1。内皮细胞通透性随年龄增长而增加23%（r = 0.44，P < 0.001）。角膜中央厚度和内皮泵率无明显变化，尽管眼压升高25%，但房水流量仍随年龄增长而保持稳定（r = 0.50，P < 0.001）。角膜基质中荧光素的荧光偏振度随年龄增长而降低（r =-0.46，P < 0.001）。我们的结论是，随着年龄的增长，人类角膜内皮细胞变得形态不规则，可能会变得更容易渗透荧光素。
Fluorophotometry with topically applied fluorescein and endothelial cell photography were performed on 80 normal subjects (age 5-79 yr). Variations in endothelial cell morphology and function, flow of aqueous humor, and intraocular pressure were recorded. The mean endothelial cell size was 332.3 .+-. 46.3 .mu.m2. A 28% increase in endothelial cell size was measured over the eight decades (r = 0.53, P < 0.001). The coefficient of variation of cell size also increased with age (r = 0.41, P < 0.001). The percentage of hexagonal endotheial cells decreased by 14% (r = -0.48, P < 0.001), while the percentage of pentagonal and heptagonal cells increased by 50% (r = 0.44, P < 0.001) and 40% (r = 0.33, P < 0.002), respectively, with age. The mean endothelial permeability to fluorescein was 4.03 .+-. 0.63 .times. 10-4 cm min-1. A 23% increase in endothelial permeability with age was observed (r = 0.44, P < 0.001). No change in central corneal thickness or endothelial pump rate was found. Flow of aqueous humor remained stable with age, despite a 25% increase in intraocular pressure (r = 0.50, P < 0.001). Polarization of fluorescence of fluorescein in the corneal stroma decreased with age (r = -0.46, P < 0.001). We conclude that with age the human corneal endothelium becomes morphologically less regular and may become more permeable to fluorescein.