Effects of light deficiency on the accumulation of saikosaponins and the ecophysiological characteristics of wild Bupleurum chinense DC. in China
Effects of light deficiency on the accumulation of saikosaponins and the ecophysiological characteristics of wild Bupleurum chinense DC. in China
复制标题
缺光对野生柴胡皂苷积累及生态生理特性的影响
DOI：
10.1016/j.indcrop.2017.01.040
复制
发表时间：
2017-05-01
影响因子：
5.9
通讯作者：
Wang, Yihui
中科院分区：
文献类型：
--
作者：
Gong, Jirui;Liu, Min;Wang, Yihui
柴胡 DC.是最重要的中药之一。在其主要药理活性成分中，柴胡皂苷起着至关重要的作用。研究了阴地和阳地林下林下低光强度下野生植物的生态生理特征、次生代谢产物的积累和抗氧化系统。与阳地植物相比，阴地植物数量较多，比叶面积（SLA）较高，最大电子传递速率（J（max））较低，磷酸丙糖利用率（TPU）较高，叶绿素b含量（Chlb）较高，但净光合作用（P-n）、根和地上生物量、暗呼吸速率（R-d）、光补偿点（LCP）、叶绿素a与b比值、类胡萝卜素含量和叶绿素b含量较低。非光化学猝灭（NPQ）。这些特征表明，在弱光条件下，该物种发育出较晚的叶子，通过光捕获复合体II捕获更多的光能，从而提高其光合效率（利用弱光的能力）和光合电子传递，从而改善碳代谢和生存。光系统II的最大量子效率在两个位点均约为0.8；因此，光抑制并不是一个主要问题。阴凉处柴胡皂苷a和d的含量以及柴胡皂苷总含量（SSa、SSd和SStotal）显着升高，同时超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）活性升高，丙二醛（MDA）降低。这表明弱光激活抗氧化应激反应途径，导致 SSa、SSd 和 SStotal 增加。我们的结果表明，B. chinense 具有很强的耐荫性，适合在农林复合系统中种植。 (C) 2017 Elsevier B.V. 保留所有权利。
Bupleurum chinense DC. is one of the most important traditional Chinese medicines. Among its primary pharmacologically active components, saikosaponins play a vital role. We investigated the ecophysiological characteristics, accumulation of secondary metabolites, and antioxidant system of wild plants at low light intensity in the forest understory at shady and sunny sites. Compared with plants at sunny sites, plants at the shady site were more abundant, had higher specific leaf area (SLA), a lower maximum electron transport rate (J(max)), higher triose phosphate utilization (TPU), and higher chlorophyll b content (Chlb), but lower net photosynthesis (P-n), root and aboveground biomass, dark respiration rate (R-d), light compensation point (LCP), chlorophyll a to b ratio, carotenoid content, and nonphotochemical quenching (NPQ). These features suggest that under low light conditions, the species developed latger leaves to catch more light energy through light -harvesting complex II to increase its photosynthetic efficiency (its ability to use weak light) and photosynthetic electron transport, thereby improving carbon metabolism and survival. The maximum quantum efficiency of photosystem II Was about 0.8 at both sites; thus, photoinhibition was not a major problem. Contents of saikosaponins a and d and the total Saikosaponin content (SSa, SSd, and SStotal) were much higher at the shady site, accompanied by increased superoxide dismutase (SOD), catalase(CAT), and peroxidases (POD) activity and decreased malondialdehyde (MDA). This suggested that low light activates antioxidant stress -response pathways, leading to increased SSa, SSd, and SStotal. Our results suggest that B. chinense has strong shade tolerance and would be suitable for cultivation in an agroforestry system. (C) 2017 Elsevier B.V. All rights reserved.